Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats.
Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats.
复制标题
对大鼠基质室的纹状体投射神经元的皮质输入的差异组织。
DOI：
10.3389/fnsys.2015.00051
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发表时间：
2015
影响因子：
3
通讯作者：
Reiner A
中科院分区：
文献类型：
--
作者：
Deng Y;Lanciego J;Kerkerian-Le-Goff L;Coulon P;Salin P;Kachidian P;Lei W;Del Mar N;Reiner A
在以前的研究中，我们描述了不同的组织皮质纹状体和丘脑纹状体输入的直接途径（dSPNs）和间接途径纹状体投射神经元（iSPNs）的基质室的脊柱。在目前的电子显微镜（EM）分析中，我们已经完善了对皮质轴棘与轴树突输入到两种类型的SPN的相对量的理解。值得注意的是，我们发现，个体dSPN从IT型（端脑内投射）皮质神经元接收的轴棘突触末梢大约是从PT型（锥体束投射）皮质神经元接收的轴棘突触末梢的两倍。我们还发现，PT型轴棘突触终端是约1.5倍，共同个别iSPN的IT型轴棘突触终端。总体而言，与iSPN棘相比，IT型端子接触dSPN的百分比更高，而与iSPN棘相比，PT型端子接触iSPN的百分比更高。值得注意的是，IT型轴棘突触末梢在iSPN棘上比在dSPN棘上显著更大。与轴棘输入相反，dSPN的轴树突PT型输入比iSPN的轴树突PT型输入更实质性，并且轴树突IT型输入对于两种SPN类型来说似乎是微薄的和可比的。突出的轴树突PT型输入dSPN可能会加重他们的PT型反应，和大尺寸的轴棘IT型终端上的iSPN可能会加重他们的IT型反应。使用transneuronal标签与狂犬病病毒选择性地标记皮质神经元与直接输入的dSPNs投射到黑质网状部，我们发现，输入主要来自运动皮层的上层神经元，其中IT型胞体占主导地位。SPNs的差异皮层输入可能在运动控制和运动学习中发挥关键作用。
In prior studies, we described the differential organization of corticostriatal and thalamostriatal inputs to the spines of direct pathway (dSPNs) and indirect pathway striatal projection neurons (iSPNs) of the matrix compartment. In the present electron microscopic (EM) analysis, we have refined understanding of the relative amounts of cortical axospinous vs. axodendritic input to the two types of SPNs. Of note, we found that individual dSPNs receive about twice as many axospinous synaptic terminals from IT-type (intratelencephalically projecting) cortical neurons as they do from PT-type (pyramidal tract projecting) cortical neurons. We also found that PT-type axospinous synaptic terminals were about 1.5 times as common on individual iSPNs as IT-type axospinous synaptic terminals. Overall, a higher percentage of IT-type terminals contacted dSPN than iSPN spines, while a higher percentage of PT-type terminals contacted iSPN than dSPN spines. Notably, IT-type axospinous synaptic terminals were significantly larger on iSPN spines than on dSPN spines. By contrast to axospinous input, the axodendritic PT-type input to dSPNs was more substantial than that to iSPNs, and the axodendritic IT-type input appeared to be meager and comparable for both SPN types. The prominent axodendritic PT-type input to dSPNs may accentuate their PT-type responsiveness, and the large size of axospinous IT-type terminals on iSPNs may accentuate their IT-type responsiveness. Using transneuronal labeling with rabies virus to selectively label the cortical neurons with direct input to the dSPNs projecting to the substantia nigra pars reticulata, we found that the input predominantly arose from neurons in the upper layers of motor cortices, in which IT-type perikarya predominate. The differential cortical input to SPNs is likely to play key roles in motor control and motor learning.