The siRNAs targeting the left or right terminal region of chrysanthemum stunt viroid (CSVd) sequence suppress the development of disease symptoms caused by CSVd infection of chrysanthemum, but do not suppress viroid propagation
The siRNAs targeting the left or right terminal region of chrysanthemum stunt viroid (CSVd) sequence suppress the development of disease symptoms caused by CSVd infection of chrysanthemum, but do not suppress viroid propagation
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DOI：
10.1080/14620316.2017.1402668
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发表时间：
2018-09
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通讯作者：
Hiroki Takino;M. Furuya;A. Sakuma;Sumiko Yamamoto;Saki Hirano;M. Tsuro;Tatsuya Yanagimoto;Yoshikazu Tanaka;M. Mino
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文献类型：
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作者：
Hiroki Takino;M. Furuya;A. Sakuma;Sumiko Yamamoto;Saki Hirano;M. Tsuro;Tatsuya Yanagimoto;Yoshikazu Tanaka;M. Mino
摘要 建立了含有产生小干扰 RNA (siRNA) 基因的转基因菊花植物，其序列是根据菊花矮化类病毒 (CSVd) 基因组设计的。 siRNA 有望通过沉默机制靶向并分解 CSVd RNA。将感染 CSVd 的植物作为接穗嫁接到非转基因或转基因植物的砧木上，将从砧木中伸长的轴芽生根并生长，以研究植物在温室条件下是否对 CSVd 具有抗性。感染CSVd的非转基因植物表现出严重的疾病症状，即生长发育迟缓和叶片上出现斑点状褪绿病斑，但在转基因植物中未观察到此类症状。用于监测CSVd量的实时定量逆转录PCR表明，CSVd在非转基因和转基因植物中的积累量相等。目前的结果表明，转基因植物对 CSVd 的抗性特征是由于干扰症状发展途径，而不是抑制 CSVd 复制。
ABSTRACT Transgenic chrysanthemum plants harbouring the genes producing small interfering RNAs (siRNAs) whose sequence was designed based on the chrysanthemum stunt viroid (CSVd) genome were established. The siRNAs are expected to target and decompose CSVd RNA through a silencing mechanism. The CSVd-infected plants were grafted as scions onto the stocks of either non-transgenic or transgenic plants, and axial shoots that elongated from the stocks were rooted and grown to investigate if the plants are resistant to CSVd under greenhouse conditions. The non-transgenic plants infected with CSVd showed severe disease symptoms, i.e. stunted growth and spotty chlorotic lesions on leaves, but no such symptoms were observed in the transgenic plants. The real-time quantitative reverse transcription PCR for monitoring the amounts of CSVd indicated that CSVd equally accumulated in both non-transgenic and transgenic plants. The present results suggest that the resistant feature of the transgenic plants to CSVd was due to interference with the pathway of symptom development, and not the inhibition of CSVd replication.