Expression of MicroRNAs in the Extracellular Microvesicles of Murine Osteoclasts
Expression of MicroRNAs in the Extracellular Microvesicles of Murine Osteoclasts
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DOI：
10.11223/jarde.10.142
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
T. Kagiya;M. Taira
中科院分区：
文献类型：
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作者：
T. Kagiya;M. Taira
142 简介 骨骼是最重要的口腔组织之一。它是通过骨形成和吸收之间的平衡来维持的。它由两种特殊的细胞类型不断重塑：骨形成成骨细胞和骨吸收破骨细胞。破骨细胞是抗酒石酸酸性磷酸酶（TRAP）阳性的多核巨细胞，起源于造血干细胞的单核细胞/巨噬细胞谱系。基质细胞和成骨细胞表达巨噬细胞集落刺激因子（M-CSF）和核因子-κB配体受体激活剂（RANKL），这对于破骨细胞的诱导至关重要。在组织工程方面，破骨细胞和巨噬细胞吸收支架，为骨基质形成腾出空间。因此，破骨细胞在骨再生中发挥重要作用。我们之前使用微阵列报道了破骨细胞生成过程中的 microRNA (miRNA) 表达谱。 miRNA 是大约 22 nu 的小型内源非编码 RNA 小鼠破骨细胞细胞外微泡中 MicroRNA 的表达
142 INTRODUCTION Bone is one of the most important oral tissues. It is maintained by the balance between bone formation and resorption . It is remodeled continuously by two specialized cell types: bone-forming osteoblasts and bone-resorbing osteoclasts . Osteoclasts are tartrate-resistant acid phosphatase (TRAP)-positive, multinucleated giant cells that originate from the monocyte/macrophage lineage of hematopoietic stem cells . Stromal cells and osteoblasts express macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL), which are essential for the induction of osteoclasts. In terms of tissue engineering, osteoclasts and macrophages resorb scaffolds, making space for bone matrix formation . Consequently, osteoclasts play an important role in bone regeneration. We reported previously microRNAs (miRNAs) expression profiles during osteoclastogenesis using microarrays . miRNAs are small, endogenous, noncoding RNAs of approximately 22 nuExpression of MicroRNAs in the Extracellular Microvesicles of Murine Osteoclasts