Self-Spreading Supported Lipid Bilayer Passing through Single Nanogap Structure: Effect of Position of Dyes in Lipid Molecules
Self-Spreading Supported Lipid Bilayer Passing through Single Nanogap Structure: Effect of Position of Dyes in Lipid Molecules
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穿过单纳米间隙结构的自铺展支撑脂质双层：脂质分子中染料位置的影响
DOI：
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
K. Torimitsu
中科院分区：
文献类型：
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作者：
Y. Kashimura;K. Furukawa;K. Torimitsu
我们报道了含有7-硝基-2-氧杂-1，3-二氮唑-4-基(NBD)荧光团的单脂双层膜在带有单个纳米间隙的图案化表面上的自扩散行为。我们发现，荧光团在纳米缝隙周围的行为取决于染料在脂质分子中的位置。对于酰链标记的脂类，它们穿过纳米间隙前后的荧光强度不连续地下降，而头基标记的脂类几乎没有观察到下降。一种可能的机制可以用染料在脂质双层中的重新定向来解释。已知酰基链标记的NBD荧光团在脂质双层中环回到脂质-水界面。由于这种效应使脂质分子的有效尺寸更大，当染料分子通过纳米间隙时，很容易受到空间位阻的影响，导致荧光强度降低。
We report the self-spreading behavior of single lipid bilayers containing 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) fluorophores on a patterned surface equipped with a single nanogap. We found that the behavior of the fluorophores around the nanogap depended on the position of the dyes in the lipid molecules. For acyl chain-labeled lipids, the fluorescence intensities decreased discontinuously before and after they passed through the nanogap, whereas little decrease was observed for a headgroup-labeled lipid. A possible mechanism can be explained in terms of the reorientation of dyes in a lipid bilayer. Acyl chain-labeled NBD fluorophores are known to loop back to a lipid–water interface in a lipid bilayer. Since this effect makes an effective size of the lipid molecule larger, dye molecules easily suffer from steric hindrance when they pass through a nanogap leading to the fluorescence intensity reduction.
影响因子：
2.9
作者：
Salafsky, J;Groves, JT;Boxer, SG
通讯作者：
Boxer, SG