A novel oxygenase from Pleurotus sapidus transforms valencene to nootkatone
A novel oxygenase from Pleurotus sapidus transforms valencene to nootkatone
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DOI：
10.1016/j.molcatb.2009.07.001
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发表时间：
2009-12-01
影响因子：
--
通讯作者：
Zorn, Holger
中科院分区：
文献类型：
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作者：
Fraatz, Marco A.;Riemer, Stephanie J. L.;Zorn, Holger
利用担子菌平菇的冻干物，实现了倍半萜(+)-戊烯的选择性高效烯丙基氧化合成(+)-nootkatone。用生化方法和层析及电泳法对酶活性进行了鉴定和纯化。通过质谱仪获得的多肽序列显示与各种子囊菌的加氧酶同源。根据测序结果，利用聚合酶链式反应从沙棘全基因组文库中扩增出全长编码区。该克隆序列全长1309个，开放阅读框为1191个碱基对。基于数据库检索，396个氨基酸的翻译氨基酸序列在蛋白质水平上与烟曲霉脂氧合酶和双色漆树脂氧合酶有50%的同源性，与大豆脂氧合酶-1的同源性为26%。担子菌的脂氧合酶还没有在分子水平上被表征。(C)2009爱思唯尔B.V.保留所有权利。
A selective and highly efficient allylic oxidation of the sesquiterpene (+)-valencene to (+)-nootkatone was achieved with lyophilisate of the basidiomycete Pleurotus sapidus. The responsible enzymatic activity was biochemically characterised and purified by chromatographic and electrophoretic methods. Peptide sequences obtained by mass spectrometry showed homologies to oxygenases from various ascomycetes. Based on the peptide sequences, the encoding cDNA was amplified from a cDNA library of P sapidus by PCR. The cloned sequence consisted of 1309 by with an open reading frame of 1191 bp. Based on database research, the translated amino acid sequence of 396 amino acids showed on the protein level homologies of similar to 50% to putative lipoxygenases from Aspergillus fumigatus and Laccaria bicolor as well as 26% homology to the sequence of lipoxygenase-1 from soy bean (Glycine max). A lipoxygenase from a basidiomycetous fungus has not yet been characterised on a molecular level. (C) 2009 Elsevier B.V. All rights reserved.