Optical-resolution photoacoustic microscopy with a needle-shaped beam
Optical-resolution photoacoustic microscopy with a needle-shaped beam
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DOI：
10.1038/s41566-022-01112-w
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发表时间：
2022-12-01
期刊：
影响因子：
35
通讯作者：
Wang, Lihong, V
中科院分区：
文献类型：
--
作者：
Cao, Rui;Zhao, Jingjing;Wang, Lihong, V
光学分辨率光声显微镜可以在细胞水平上可视化依赖于波长的光吸收。然而，由于光激发光束的紧密聚焦，该技术受到景深的限制，这使得获取表面不平整的样品的高分辨率图像或在不进行z扫描的情况下获得高质量的体积图像具有挑战性。为了克服这一限制，我们提出了针状光束光声显微镜，它可以通过定制的衍射光学元件将景深扩展到28倍瑞利长度左右。这些衍射光学元件产生具有良好保持的光束直径、均匀的轴向光强分布和可以忽略的旁瓣的针状光束。使用266 nm激光对新鲜的无载玻片器官进行组织学成像，以及使用532 nm激光对小鼠脑血管进行活体成像，都证明了使用针状光束光声显微镜的优势。这种方法为术中无载玻片的病理成像和活体器官水平成像提供了新的视角。
Optical-resolution photoacoustic microscopy can visualize wavelength-dependent optical absorption at the cellular level. However, this technique suffers from a limited depth of field due to the tight focus of the optical excitation beam, making it challenging to acquire high-resolution images of samples with uneven surfaces or high-quality volumetric images without z scanning. To overcome this limitation, we propose needle-shaped beam photoacoustic microscopy, which can extend the depth of field to around a 28-fold Rayleigh length via customized diffractive optical elements. These diffractive optical elements generate a needle-shaped beam with a well-maintained beam diameter, a uniform axial intensity distribution and negligible sidelobes. The advantage of using needle-shaped beam photoacoustic microscopy is demonstrated via both histology-like imaging of fresh slide-free organs using a 266 nm laser and in vivo mouse-brain vasculature imaging using a 532 nm laser. This approach provides new perspectives for slide-free intraoperative pathological imaging and in vivo organ-level imaging.