Continuous Allosteric Regulation of a Viral Packaging Motor by a Sensor that Detects the Density and Conformation of Packaged DNA
Continuous Allosteric Regulation of a Viral Packaging Motor by a Sensor that Detects the Density and Conformation of Packaged DNA
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DOI：
10.1016/j.bpj.2014.11.3469
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发表时间：
2015-01-20
影响因子：
3.4
通讯作者：
Smith, Douglas E.
中科院分区：
文献类型：
--
作者：
Berndsen, Zachary T.;Keller, Nicholas;Smith, Douglas E.
我们报告了一种非常规的生物运动变构调节的证据。我们表明，噬菌体phi 29的基因组包装电机是由一个传感器，检测包装在病毒衣壳内的DNA的密度和构象进行调节，并减慢电机的直接负载力的效果不同的机制。具体来说，我们表明，电机ATP相互作用的信号，传播变构从病毒外壳内的电机安装在外面。该信号响应于包装DNA的密度或构象的变化而连续地调节马达速度和暂停，并且在抵抗DNA限制的大的力积累之前减慢马达。对电机打滑的分析表明，包装的阻力仍然很低（
We report evidence for an unconventional type of allosteric regulation of a biomotor. We show that the genome-packaging motor of phage phi 29 is regulated by a sensor that detects the density and conformation of the DNA packaged inside the viral capsid, and slows the motor by a mechanism distinct from the effect of a direct load force on the motor. Specifically, we show that motor-ATP interactions are regulated by a signal that is propagated allosterically from inside the viral shell to the motor mounted on the outside. This signal continuously regulates the motor speed and pausing in response to changes in either density or conformation of the packaged DNA, and slows the motor before the buildup of large forces resisting DNA confinement. Analysis of motor slipping reveals that the force resisting packaging remains low (