Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates
Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates
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DOI：
10.1016/j.biomaterials.2007.02.022
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发表时间：
2007-07-01
期刊：
影响因子：
14
通讯作者：
Gristina, R.
中科院分区：
文献类型：
--
作者：
Buttiglione, M.;Vitiello, F.;Gristina, R.
在神经元细胞培养实验中可以测试的参数中，使用不同的培养基和基质代表了影响细胞粘附和分化的强大分析。在这项工作中，等离子体增强化学气相沉积（PE-CVD）从丙烯酸和烯丙胺蒸汽进行，沉积涂层含有氧(0)和氮(N)的官能团在聚醚聚酸酯（PET）表面。人神经母细胞瘤SH-SY5Y细胞在血浆修饰的底物和含有不同量的胎牛血清（FCS）的培养基或含cAMP的无血清培养基中生长。结果表明，含氮基质能促进细胞黏附，而细胞培养基对神经突的萌发有影响。有趣的是，在含血清培养基中生长的细胞中，修饰表面羧基的存在可以影响分化标志物神经丝-200 （NF-H）的表达。(C) 2007 Elsevier Ltd.版权所有。
Among the parameters that can be tested in experiments on neuronal cell culture the use of different culture media and substrates represents a powerful assay to influence cell adhesion and differentiation. In this work, plasma-enhanced-chemical vapour depositions (PE-CVD) from acrylic acid and allylamine vapours have been performed to deposit coatings bearing oxygen (0)- and nitrogen (N)-containing functional groups on polyethylenethereplualate (PET) surface. Human neuroblastoma SH-SY5Y cells were grown on plasma modified substrates and in presence of media containing different amount of fetal calf serum (FCS) or in serum-free medium containing cAMP. Our results showed that N-containing substrates improved cell adhesion, while the neurites sprouting was influenced by cell culture media. Interestingly, the presence of carboxylic groups on the modified surface can influence the expression of a differentiation marker, neurofilament-200 (NF-H), in cells grown in serum-containing media. (C) 2007 Elsevier Ltd. All rights reserved.