Biomimetic Silk Fibroin Hydrogels Strengthened by Silica Nanoparticles Distributed Nanofibers Facilitate Bone Repair
Biomimetic Silk Fibroin Hydrogels Strengthened by Silica Nanoparticles Distributed Nanofibers Facilitate Bone Repair
复制标题
由二氧化硅纳米粒子分布式纳米纤维增强的仿生丝素蛋白水凝胶有利于骨修复。
DOI：
10.1002/adhm.202001646
复制
发表时间：
2021-03-10
影响因子：
10
通讯作者：
Li, Zubing
中科院分区：
文献类型：
--
作者：
Cheng, Yuet;Cheng, Gu;Li, Zubing
各种材料被用作骨修复的人工替代物。在这项研究中，制备了一种由短二氧化硅纳米颗粒（SiNPs）分布的丝素蛋白纳米纤维（SiNPs@ NF）增强的丝素蛋白（SF）水凝胶，该水凝胶具有上级骨诱导性能，用于治疗骨缺损。SF作为复合支架的基础部分以模拟细胞外基质（ECM），其是天然骨的有机组分。SiNP簇在复合水凝胶内的分布部分模拟了ECM内矿物晶体的分布。SiNPs@ NF的掺入增强了复合水凝胶的机械性能。此外，该复合水凝胶在体外为细胞粘附、增殖和成骨分化提供了生物相容性微环境。体内研究证实，在用复合水凝胶治疗的大尺寸颅骨缺损中形成大量新骨，实现了成功的修复。总之，本研究中制备的SiNPs@ NF增强水凝胶具有用于骨组织工程的潜力。
Various materials are utilized as artificial substitutes for bone repair. In this study, a silk fibroin (SF) hydrogel reinforced by short silica nanoparticles (SiNPs)-distributed-silk fibroin nanofibers (SiNPs@NFs), which exhibits a superior osteoinductive property, is fabricated for treating bone defects. SF acts as the base part of the composite scaffold to mimic the extracellular matrix (ECM), which is the organic component of a native bone. The distribution of SiNPs clusters within the composite hydrogel partially mimics the distribution of mineral crystals within the ECM. Incorporation of SiNPs@NFs enhances the mechanical properties of the composite hydrogel. In addition, the composite hydrogel provides a biocompatible microenvironment for cell adhesion, proliferation, and osteogenic differentiation in vitro. In vivo studies confirm that the successful repair is achieved with the formation of a large amount of new bone in the large-sized cranial defects that are treated with the composite hydrogel. In conclusion, the SiNPs@NFs-reinforced-hydrogel fabricated in this study has the potential for use in bone tissue engineering.