Differential Effects of Phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on Stomatal Responses to Environmental Signals.
Differential Effects of Phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on Stomatal Responses to Environmental Signals.
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DOI：
10.3389/fpls.2017.00677
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发表时间：
2017
影响因子：
5.6
通讯作者：
Iba K
中科院分区：
文献类型：
--
作者：
Takahashi S;Monda K;Higaki T;Hashimoto-Sugimoto M;Negi J;Hasezawa S;Iba K
细胞内的磷脂酰肌醇（PI）代谢产物作为信号分子在气孔对环境信号的响应中起着重要作用。在这项研究中，一组细胞成分，包括PI 4-激酶（PI 4K）和PI 3 K的药理学抑制剂，被用来调查气孔关闭响应CO2，黑暗，和脱落酸（阿坝）。与黑暗和阿坝处理相比，PAO（一种PI 4K的特异性抑制剂）处理特异性地抑制了气孔对CO2的反应。与此相反，与CO2和阿坝相比，PI 3 K特异性抑制剂LY 294002处理特异性抑制气孔对黑暗的反应。PAO和LY 294002的特异性抑制作用也被观察为由绿色荧光蛋白标记的PATROL 1标记的点状结构的空间密度的变化，PATROL 1是一种可能通过调节保卫细胞质膜中H+-ATP酶量来控制气孔开度的蛋白质。我们的研究结果表明，PI 4K和PI 3 K在CO2和黑暗信号转导途径中分别发挥重要作用，介导PATROL 1动力学。
Specific cellular components including products of phosphatidylinositol (PI) metabolism play an important role as signaling molecules in stomatal responses to environmental signals. In this study, pharmacological inhibitors of a set of cellular components, including PI4-kinase (PI4K) and PI3K, were used to investigate stomatal closure in response to CO2, darkness, and abscisic acid (ABA). Treatment with PAO, a specific inhibitor of PI4K, specifically inhibited the stomatal response to CO2 compared with that to darkness and ABA. In contrast, treatment with LY294002, a PI3K-specific inhibitor, specifically inhibited the stomatal response to darkness compared with that to CO2 and ABA. The specific inhibitory effects of PAO and LY294002 were also observed as changes in the spatial density of dot-like structures labeled by green fluorescent protein-tagged PATROL1, a protein that controls stomatal aperture possibly via regulation of H+-ATPase amount in guard cell plasma membranes. Our results suggest an important role for PI4K and PI3K in the CO2 and darkness signal transduction pathways, respectively, that mediate PATROL1 dynamics.