Infrared laser-assisted desorption electrospray ionization mass spectrometry
Infrared laser-assisted desorption electrospray ionization mass spectrometry
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DOI：
10.1039/b715146b
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发表时间：
2008-01-01
期刊：
影响因子：
4.2
通讯作者：
Murray, Kermit K.
中科院分区：
文献类型：
--
作者：
Rezenom, Yohannes H.;Dong, Jianan;Murray, Kermit K.
我们已经使用了红外激光解吸的材料和电离的相互作用与电喷雾溶剂。红外激光辅助解吸电喷雾电离（IR LADESI）质谱法用于在环境条件下直接分析含水样品。离子阱质谱仪进行了修改，包括在2.94 μ m波长的脉冲Er：YAG激光耦合到氧化锗光纤解吸在大气压和纳米电喷雾源电离。将水溶液中的分析物置于不锈钢靶上，并用脉冲IR激光照射。从目标解吸和烧蚀的材料被来自电喷雾溶剂的带电液滴的连续流电离。使用这种方法分析的肽和蛋白质样品产生的质谱与通过常规电喷雾获得的质谱相似。在没有样品预处理的情况下分析血液和尿液，以证明IR LADESI直接分析生物液体的能力。还直接分析了药品。最后讨论了水作为基体在IRLADESI过程中的作用。
We have used an infrared laser for desorption of material and ionization by interaction with electrosprayed solvent. Infrared laser-assisted desorption electrospray ionization (IR LADESI) mass spectrometry was used for the direct analysis of water-containing samples under ambient conditions. An ion trap mass spectrometer was modified to include a pulsed Er: YAG laser at 2.94 mu m wavelength coupled into a germanium oxide optical fiber for desorption at atmospheric pressure and a nanoelectrospray source for ionization. Analytes in aqueous solution were placed on a stainless steel target and irradiated with the pulsed IR laser. Material desorbed and ablated from the target was ionized by a continuous stream of charged droplets from the electrosprayed solvent. Peptide and protein samples analyzed using this method yield mass spectra similar to those obtained by conventional electrospray. Blood and urine were analyzed without sample pretreatment to demonstrate the capability of IR LADESI for direct analysis of biological fluids. Pharmaceutical products were also directly analyzed. Finally, the role of water as a matrix in the IR LADESI process is discussed.