Relative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep
Relative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep
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DOI：
10.1016/j.anireprosci.2020.106347
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发表时间：
2020-05-01
影响因子：
2.2
通讯作者：
Atli, Mehmet O.
中科院分区：
文献类型：
--
作者：
Alak, Ilyas;Hitit, Mustafa;Atli, Mehmet O.
本研究旨在探讨ISG15 mRNA转录本在着床前和着床期间子宫内（滋养细胞、子宫内膜）和子宫外（下丘脑、垂体前叶、黄体）组织中的相对丰度和定位。在发情后第12天和第16天，将多产母羊随机分为妊娠或发情周期4组（每组n = 4）。采用实时定量PCR检测ISG15 mRNA转录物在子宫内膜、黄体、下丘脑和垂体前叶中的相对丰度。利用原位杂交技术评估ISG15 mRNA转录物的定位。ISG15 mRNA转录本仅在妊娠第12天在子宫内膜和滋养细胞等宫内组织中可见。妊娠第16天所有组织中均检测到ISG15 mRNA转录物。妊娠第12天子宫内膜中ISG15 mRNA转录物的丰度高于其他时间，而在妊娠第16天除下丘脑外的所有其他组织中，ISG15 mRNA的丰度都很高。由此可见，ISG15 mRNA转录本仅在着床前存在于子宫内组织中。然而，ISG15 mRNA转录物在着床期间存在于母羊的子宫外组织中，这可能是由于胚胎发育过程中产生的血液循环中干扰素-tau含量的增加。研究结果还首次表明，妊娠与母羊下丘脑内和垂体前叶ISG15 mRNA转录物丰度增加有关。
The aim of this study was to investigate relative abundance and localization of ISG15 mRNA transcript in intra-uterine (trophoblast, endometrium) and extra-uterine (hypothalamus, anterior pituitary, corpus luteum) tissues before and during the period of conceptus implantation. Multiparous ewes (n = 16) were randomly allotted into four groups: pregnant or estrous cyclic on days of 12 and 16 (n = 4 per group) following estrus. Relative abundances of ISG15 mRNA transcript were determined in the endometrium, corpus luteum, hypothalamus, and anteriorpituitary using real time quantitative PCR. Localization of ISG15 mRNA transcript was evaluated using in situ hybridization. The presence of ISG15 mRNA transcript was only visualized in intrauterine tissues including the endometrium and trophoblast on day 12 of pregnancy. The ISG15 mRNA transcript was detected in all tissues evaluated on day 16 of pregnancy. The abundance of ISG15 mRNA transcript was greater in the endometrium on day 12 of pregnancy than at other days when evaluations occurred while in all other tissues except the hypothalamus there were large abundances of ISG15 mRNA on day 16 of pregnancy. It is concluded that the ISG15 mRNA transcript is only present in intra-uterine tissues before conceptus implantation. The ISG15 mRNA transcript, however, is present in extra-uterine tissues of ewes during implantation probably due to an increased amount of interferon-tau in blood circulation that is produced by the developing embryo. Results also indicate, for the first time, that pregnancy is associated with an intra-hypothalamus and anterior pituitary increased abundance of ISG15 mRNA transcript in ewes.