Effects of Circular RNA of Chicken Growth Hormone Receptor Gene on Cell Proliferation.
Effects of Circular RNA of Chicken Growth Hormone Receptor Gene on Cell Proliferation.
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DOI：
10.3389/fgene.2021.598575
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发表时间：
2021
影响因子：
3.7
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Xu H;Leng Q;Zheng J;Adu-Asiamah P;Lin S;Li T;Wang Z;An L;Zhao Z;Zhang L
动物的生长发育受神经和内分泌生长轴的调控，其中细胞增殖起着关键作用。近年来，大量研究表明环状RNA参与了肝细胞和成肌细胞的增殖。在此之前，我们发现了一个来自鸡GHR基因的环状RNA，命名为circGHR。然而，circGHR的功能尚不清楚。本研究的目的是研究circGHR的表达模式及其在细胞增殖中的作用。结果表明，circGHR是一种闭环结构分子，在肝细胞和成肌细胞的细胞核中含量较高。实时荧光定量PCR结果显示，从E13到第7周，肝脏中的circGHR增加，而大腿和胸肌中的circGHR降低。CCK-8法显示circGHR促进细胞增殖。同时，当circGHR过表达时，生物标志物基因PCNA、CCND 1和CDK 2以及线性转录物GHR和GHBP上调。综上所述，circGHR可能通过调控GHR mRNA和GHBP的表达而促进细胞增殖。
Animal growth and development are regulated by neural and endocrine growth axes, in which cell proliferation plays key roles. Recently, many research showed that circular RNAs were involved in hepatocyte and myoblast proliferation. Previously, we identified a circular RNA derived from the chicken GHR gene, named circGHR. However, the function of circGHR is unclear. The objective of this study was to investigate circGHR expression pattern and its roles in cell proliferation. Results indicated that circGHR was a closed-loop structure molecule, and it was richer in the nucleus of hepatocytes and myoblast. Real-time PCR showed that circGHR was increased from E13 to the 7th week in the liver but decreased in the thigh and breast muscle. The CCK-8 assay displayed that circGHR promoted cell proliferation. Simultaneously, the biomarker genes PCNA, CCND1, and CDK2 and the linear transcripts GHR and GHBP were upregulated when circGHR was overexpressed. Altogether, these data exhibited that circGHR could promote cell proliferation possibly by regulating GHR mRNA and GHBP expression.