The macroalgal culture collection in Kobe University (KU-MACC), and a comprehensive molecular phylogeny of macroalgae based on the culture strains.
The macroalgal culture collection in Kobe University (KU-MACC), and a comprehensive molecular phylogeny of macroalgae based on the culture strains.
复制标题
神户大学大型藻类培养物保藏中心（KU-MACC），以及基于培养菌株的大型藻类综合分子系统发育。
DOI：
10.1080/26388081.2020.1745685
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发表时间：
2020
影响因子：
--
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Kawai;H.;Hanyuda;T.;Akita;S.;Uwai;S.
神户大学大型藻类培养库(KU-MACC)作为神户大学内陆海研究中心的一个分支，在文部科学省国家生物资源项目和日本医学研究开发机构的保护下，与国家环境研究所的微生物培养库合作，向公众分发大型藻类培养物。Ku-MACC主要含有大型藻类(裸藻、红藻和石藻)，但也有一些与裂藻和轮藻相关的重要类群。它们是单藻菌株，以营养菌体的形式保存，并通过冷冻保存。菌种在不同温度条件下的培养箱中培养，培养瓶内装有普罗瓦索利浓缩海水(PES)或PESI培养液。收集的“基因组菌株”包括Etocarpus silicosus、Chondrus cruspus、Chara braunii以及它们的相关菌株。根据系统发育类群的不同，核rDNA ITS区、叶绿体bc1和线粒体3基因的DNA序列被确定并用于分类学和遗传标记。根据遗传数据，提出了石藻、褐藻和红藻的分子系统学关系。在石藻亚科的分子系统发育研究中，由于培养菌株的覆盖率较低，KU-MACC菌株只代表Ulothricales、Ulvales和Bryopsidales的成员，而该纲有10个目。基于cox3基因的褐藻科分子系统发育与已发表的多基因分子系统发育树结构相似，但大多数节点的引导支持度较低：在多基因分子系统发育树中，石杉属在棕榈科的底部附近分枝，与双孢子菌目相邻，但在coox3树中，其分支较长，与棕榈科是姊妹种。在红藻门的分子系统发育中，KU-MACC菌株代表了14个目，涵盖了目前已知的约一半的班吉藻和氟藻目。
The Kobe University Macroalgal Culture Collection (KU-MACC), as a section of the Kobe University Research Center for Inland Seas, distributes cultures of macroalgae to the public under the umbrella of the National BioResource Project of Ministry of Education, Culture, Sports, Science and Technology and Japan Agency for Medical Research and Development, in collaboration with the Microbial Culture Collection of the National Institute of Environmental Studies. KU-MACC principally holds macroalgae (Phaeophyceae, Rhodophyta and Ulvophyceae), but also holds some important related taxa of Schizocladiophyceae and Charophyceae. They are unialgal strains maintained as vegetative thalli and by cryo-preservation. Stock cultures are grown in culture chambers under various temperature conditions in transparent polypropylene vials containing Provasoli’s Enriched Seawater (PES) or PESI medium. The collection includes the “genome strains” ofEctocarpus siliculosus, Chondrus crispus, Chara brauniiand their related stains. Depending on the phylogenetic group, DNA sequences of the nuclear rDNA ITS region, chloroplastrbcL, and mitochondrialcox3 genes are determined and used for taxonomy and as genetic tags. Based on the genetic data, molecular phylogenies of Ulvophyceae, Phaeophyceae and Rhodophyta are presented. In therbcL molecular phylogeny of Ulvophyceae, due to rather limited coverage of culture strains, only members of Ulothricales, Ulvales and Bryopsidales are represented by KU-MACC strains, whereas 10 orders are recognized in the class. The molecular phylogeny of Phaeophyceae based on thecox3 gene showed a tree topology similar to the published multigene molecular phylogeny except for the position ofIshige okamurae, although the bootstrap supports of most nodes were low: Ishigeales branched near the base of Phaeophyceae next to Discosporangiales in the multigene molecular phylogeny, but in thecox3 tree, it showed a long branch and was sister to Fucales. In the molecular phylogeny of Rhodophyta, 14 orders are represented by KU-MACC strains covering about half of currently recognized Bangiophycean and Florideophycean orders.