Effects of galanin-like peptide (GALP) on locomotion, reproduction, and body weight in female and male mice
Effects of galanin-like peptide (GALP) on locomotion, reproduction, and body weight in female and male mice
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DOI：
10.1016/j.yhbeh.2005.01.010
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发表时间：
2005-08-01
影响因子：
3.5
通讯作者：
Rissman, EF
中科院分区：
文献类型：
--
作者：
Kauffman, AS;Buenzle, J;Rissman, EF
甘丙肽样肽（GALP）参与摄食和生殖的神经内分泌调节。在雄性啮齿类动物和灵长类动物中，脑室内（icv）输注GALP刺激促黄体生成激素（LH）释放，诱导与摄食和生殖有关的脑区中的Fos表达，并影响啮齿类动物的摄食量和体重。在性腺完整和去势的雄性大鼠中，icv给予GALP也刺激雄性性行为。虽然GALP对男性生理和行为的影响有很好的记录，但没有研究涉及GALP在女性中的作用。我们测试了icv GALP输注对成年卵巢切除雌性小鼠LH释放、自发活动、运动控制和体重调节的影响，这些小鼠用苯甲酸雌二醇和孕酮预先刺激。此外，性经验的雄性和雌性小鼠用GALP处理并测试性行为。在雌性动物中，GALP以剂量依赖性方式降低了旷场运动活动、在加速旋转杆上保持抓握的能力和24小时体重。GALP还增加了雌性小鼠的LH分泌，这种作用可通过Antide（一种促性腺激素释放激素（GnRH）1型受体拮抗剂）预处理来阻断。GALP输注略微降低了雌性小鼠脊柱前凸行为的发生率，并显著增加了雌性小鼠表现出接受性的乳酸。与之前在雄性大鼠中的报道不同，GALP抑制小鼠的雄性性行为。我们的数据表明，在雌性小鼠中，GALP通过GnRH刺激LH释放，并通过GnRH非依赖性途径降低体重，运动控制和运动活动。此外，我们的性行为和运动的研究结果表明，在大鼠和小鼠的大脑中的GALP作用的机制和/或位置的物种特异性差异。(c)2005年爱思唯尔公司All rights reserved.
Galanin-like peptide (GALP) has been implicated in the neuroendocrine regulation of both feeding and reproduction. In male rodents and primates, intracerebroventricular (icv) infusions of GALP stimulate luteinizing hormone (LH) release, induce Fos expression in brain areas implicated in feeding and reproduction, and affect food intake and body weight in rodents. In gonad-intact and castrated male rats, icv administration of GALP also stimulates male sexual behavior. While the effects of GALP on male physiology and behavior are well documented, no studies have addressed such a role of GALP in females. We tested the effects of icv GALP infusions on LH release, locomotor activity, motor control, and body weight regulation in adult ovariectomized female mice hormonally primed with estradiol benzoate and progesterone. In addition, sexually-experienced male and female mice were treated with GALP and tested for sexual behavior. In females, GALP reduced open-field locomotor activity, the ability to maintain grip on an accelerating rotarod, and 24-h body weight in a dose-dependent manner. GALP also increased LH secretion in female mice, an effect that was blocked by pre-treatment with Antide, a gonadotropin-releasing hormone (GnRH) type-1 receptor antagonist. GALP infusions slightly decreased the occurrence of lordosis behavior in female mice and significantly increased the latencies with which females displayed receptivity. Unlike previous reports in male rats, GALP inhibited male sexual behavior in mice. Our data indicate that in female mice, GALP stimulates LH release via GnRH, and decreases body weight, motor control, and locomotor activity via GnRH-independent pathways. Furthermore, our sexual behavior and locomotor findings suggest species-specific differences in the mechanism and/or location of GALP action in the brains of rats and mice. (c) 2005 Elsevier Inc. All rights reserved.