PURIFICATION AND CHARACTERIZATION OF A LECTIN (PLANT HEMAGGLUTININ) WITH BLOOD GROUP-A SPECIFICITY FROM DOLICHOS-BIFLORUS
PURIFICATION AND CHARACTERIZATION OF A LECTIN (PLANT HEMAGGLUTININ) WITH BLOOD GROUP-A SPECIFICITY FROM DOLICHOS-BIFLORUS
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DOI：
10.1021/bi00806a022
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发表时间：
1970-01-01
期刊：
影响因子：
2.9
通讯作者：
KABAT, EA
中科院分区：
文献类型：
--
作者：
ETZLER, ME;KABAT, EA
玛丽莲·E Etzlerf和Elvin A. Kabat摘要：用不溶性多亮氨酰A血型物质吸附，再用2-脱氧-/3-D-吡喃半乳糖苷（D-GalNAc）洗脱，从白扁豆种子中纯化出A血型特异性凝集素。纯化的凝集素是均匀的超离心，在丙烯酰胺凝胶电泳在酸性和碱性条件下，给一个扩散带，形成一条线的免疫扩散和免疫电泳对兔抗血清的粗种子提取物，并完全沉淀的人血型A物质，它有聚集在溶液中的趋势。氨基酸分析表明，纯化的凝集素中含有大量的天冬氨酸和丝氨酸，但没有半胱氨酸或蛋氨酸。凝集素含有约2%的己糖，分子量为140，000，等电点为pH 4.5。凝集素与血型A1和A2物质以及与链球菌C组多糖沉淀。这些多糖中的每一种的反应性是-V^拉尼植物含有能够与动物红细胞和其他细胞特异性结合的凝集素（关于综述，参见Kriipe，1956，Makela，1957和Bird，1959）。这些血凝素，称为凝集素（Boyd和Shapleigh，1954 b），最常在豆科植物的种子中发现，它们的特异性彼此不同，其中某些是针对ABH血型物质的（参见图1）。Makela，1957）。
Marilynn E. Etzlerf and Elvin A. Kabat abstract: The blood group A specific lectin from Dolichos bifiorus seeds was purified by adsorption on to insoluble polyleucyl blood group A substance and subsequent elution with 2-deoxy-/3-D-galactopyranoside (D-GalNAc). The purified lectin was homogeneous by ultracentrifugation, gave one diffuse band on acrylamide gel electrophoresis under acid and alkaline conditions, formed one line in immunodiffusion and immunoelectrophoresis against rabbit antisera to the crude seed extract, and was totally precipitated by human blood group A substance; it had a tendency to aggregate in solution. Amino acid analyses of the purified lectin showed a large amount of aspartic acid and serine but no cysteine or methionine. The lectin contains about 2% hexose and has a molecular weight of 140,000 and an isoelectric point of pH 4.5. The lectin precipitated with blood group Ai and A2 substances as well as with the streptococcal group C poly-saccharide. The reactivity of each of these polysaccharides is-^ V^ Lany plants contain agglutinins capable of combining specifically with animal erythrocytes and other cells (for reviews, see Kriipe, 1956, Makela, 1957, and Bird, 1959). These hemagglutinins, called lectins (Boyd and Shapleigh, 1954b), are most frequently foundin the seeds of leguminous plants and differfrom one another in their specificities, cer-tain of which are directed toward the blood group ABH substances (cf. Makela, 1957).