Proteoglycan metabolism associated with mouse metanephric development: morphologic and biochemical effects of beta-D-xyloside.
Proteoglycan metabolism associated with mouse metanephric development: morphologic and biochemical effects of beta-D-xyloside.
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与小鼠后肾发育相关的蛋白多糖代谢：β-D-木糖苷的形态和生化作用。
DOI：
10.1016/0012-1606(87)90388-5
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发表时间：
1987
影响因子：
2.7
通讯作者：
Klein,DJ
中科院分区：
文献类型：
--
作者：
Platt,JL;Brown,DM;Granlund,K;Oegema,TR;Klein,DJ
在器官发生开始时，在胎鼠肾脏中研究了形态学和[35 S]硫酸盐重新掺入蛋白聚糖中的情况。器官培养和活体内分支形态发生和肾单位发育与软骨素-SO 4和乙酰肝素-SO 4蛋白聚糖的新生合成有关。通过分析对硝基苯基-β-d-木糖苷（β-d-xyloside，β-d-xyloside）对肾脏发育和蛋白多糖代谢的影响，探讨蛋白多糖代谢在后肾发生中的作用。在1.0和0.5mM浓度的β-d-木糖苷中孵育胎肾，但在0.1mM浓度下不孵育，引起输尿管分支的抑制，并显著减少Mr2.0 × 106 Da的软骨素-SO_4蛋白聚糖的合成。在所有β-d-木糖苷浓度下，[35 S]硫酸盐的掺入都受到刺激，反映了木糖苷引发的皮肤素-35SO4链的合成。与对硫酸软骨素合成和输尿管分支的显著影响相反，β-d-木糖苷对硫酸乙酰肝素合成或肾小球和肾小球基底膜的发育没有影响。因此，我们的特点蛋白多糖合成过程中的早期肾器官和描述的意见，这表明之间的关联软骨素-SO 4蛋白多糖的代谢和输尿管的发展。
Morphology andde novoincorporation of [35S]sulfate into proteoglycans were studied in fetal mouse kidneys at the onset of organogenesis. Branching morphogenesis and nephron development in organ culture andin vivowere associated withde novosynthesis of chondroitin-SO4and heparan-SO4proteoglycans. The role of proteoglycan metabolism in metanephrogenesis was then studied by analysis of the effects ofp-nitrophenyl-β-d-xylopyranoside (β-d-xyloside) on renal development and proteoglycan metabolism. Incubation of fetal kidneys in β-d-xyloside at concentrations of 1.0 and 0.5 mM, but not at 0.1 mM, caused inhibition of ureteric branching and markedly diminished synthesis of a largeMr2.0 × 106Da chondroitin-SO4proteoglycan. Incorporation of [35S]sulfate was stimulated at all β-d-xyloside concentrations, reflecting synthesis of xyloside initiated dermatan-35SO4chains. In contrast to dramatic effects on chondroitin-SO4synthesis and ureteric branching, β-d-xyloside had no effect on heparan-SO4synthesis or on development of the glomerulus and glomerular basement membrane. We thus characterize the proteoglycans synthesized early in the course of renal organogenesis and describe observations which suggest an association between metabolism of chondroitin-SO4proteoglycan and development of the ureter.