NITRATE-REDUCING AND NITRITE-REDUCING BACTERIA IN THE ACHLORHYDRIC STOMACH
NITRATE-REDUCING AND NITRITE-REDUCING BACTERIA IN THE ACHLORHYDRIC STOMACH
复制标题
DOI：
10.1099/00222615-25-4-253
复制
发表时间：
1988-04-01
影响因子：
3
通讯作者：
COLE, JA
中科院分区：
文献类型：
--
作者：
FORSYTHE, SJ;DOLBY, JM;COLE, JA
采用好氧和严格厌氧培养技术测定了8例胃液的微生物组成。共分离出16属细菌，但每位患者仅分离出3属代表性细菌（链球菌、奈瑟菌和血友病菌）。细孔菌、血友病菌、葡萄球菌、杆状菌、乳酸菌、黄杆菌和梭杆菌都能还原硝酸盐和亚硝酸盐，但细孔菌、副流感嗜血杆菌和肠杆菌科成员的混悬液对硝酸盐的还原速度比亚硝酸盐的还原速度快10倍。相反，虽然所有奈瑟菌都能还原亚硝酸盐，但只有部分菌株能还原硝酸盐。链球菌没有降低硝酸盐。血链球菌在与血红蛋白一起生长时减少亚硝酸盐；其他链球菌没有减少亚硝酸盐。在pH值6 ~ 8范围内，细菌的硝酸盐和亚硝酸盐还原活性较强，与胃液的pH值范围相似。根据细菌菌群的组成及其在普遍条件下硝酸盐和亚硝酸盐的潜在还原速率，对硝酸盐还原过程中亚硝酸盐的积累趋势进行了预测。对副流感嗜血杆菌和亚黄嗜血杆菌混合培养的亚硝酸盐瞬时积累的研究与这些预测一致。血友病菌和细孔菌可能是一些患者胃液中亚硝酸盐积累的原因，而链球菌和奈瑟菌则倾向于在亚硝酸盐形成时迅速将其从胃中清除。
The microbial composition of samples of gastric juice from eight achlorhydric patients was determined by aerobic and rigorously anaerobic culture techniques. Bacteria from 16 genera were commonly isolated, but representatives of only three genera, (streptococci, neisseriae and haemophili) were isolated from every patient. Nitrate and nitrite were both reduced by veillonellae, haemophili, staphylococci, corynebacteria, lactobacilli, flavobacteria and fusobacteria, but the potential rate of nitrate reduction by suspensions of veillonellae, Haemophilus parainfluenzae and members of the Enterobacteriaceae were up to ten times more rapid than the rate of nitrite reduction. Conversely, although all Neisseria spp. reduced nitrite only some strains reduced nitrate. Streptococci did not reduce nitrate. Streptococcus sanguis reduced nitrite when grown with haematin; other streptococci did not reduce nitrite. Bacterial nitrate and nitrite reduction were active over the pH range 6-8, similar to the pH range of the achlorhydric stomach. From a knowledge of the composition of the bacterial flora and their potential rates of nitrate and nitrite reduction under prevailing conditions, predictions were made about the tendency of nitrite to accumulate during nitrate reduction. Studies of the transient accumulation of nitrite by mixed cultures of H. parainfluenzae and N. subflava were consistent with these predictions. Haemophili and veillonellae could be responsible for the accumulation of nitrite in the gastric juice of some patients, whereas streptococci and neisseriae would tend to remove nitrite from the stomach as rapidly as it formed.