Salivary testosterone determination in studies of child health and development
Salivary testosterone determination in studies of child health and development
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DOI：
10.1006/hbeh.1998.1492
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发表时间：
1999-02-01
影响因子：
3.5
通讯作者：
Arentz, M
中科院分区：
文献类型：
--
作者：
Granger, DA;Schwartz, EB;Arentz, M
由于在发育研究中有许多潜在的应用，儿童唾液中激素的测量引起了人们的兴趣。虽然儿童唾液中某些激素（如皮质醇）的测定方法已被广泛使用，但儿童唾液睾酮测定方法的可用性和使用受到限制。通过采用市售的血清睾酮试剂盒，我们的实验室开发了一种可靠、高效、高灵敏度的方法来测量儿童唾液中的睾酮，不需要分离或提取。最低检出限为0.8 pg/mL。浓度为9.25 ~ 86.41 pg/mL时，测定内变异系数（CV）在3.66 ~ 6.78%之间；浓度为7.3 ~ 118.51 pg/mL时，测定间变异系数（CV）在5.70 ~ 6.61%之间。标准曲线重现性好（M斜率= -0.70,M-r = 0.99）。方法精密度为99.20%，线性度为92.80%。匹配的血清和唾液样本的值显示出很强的线性关系。该分析捕获了男孩（N = 90）和女孩（N = 85）、8-12岁、AM和PM唾液睾酮水平个体差异范围的近99.09%。这种分析可以很容易地应用于研究儿童健康和发展的背景下的睾酮行为关系的调查。它可以帮助许多儿童发展研究人员改进或扩大他们的研究活动。(C) 1999学术出版社。
Measurement of hormones in children's saliva has excited interest because of numerous potential applications in developmental studies. Although assays of children's saliva for some hormones (e.g., cortisol) are widely available and used, the availability and use of assays of children's saliva testosterone is restricted. By adapting a commercially available serum testosterone kit, our laboratory has developed a reliable, efficient, and highly sensitive procedure for measuring testosterone in children's saliva that does not require separation or extraction. The minimum detection limit was 0.8 pg/mL. Intraassay coefficients of variation (CV) were between 3.66 and 6.78% at concentrations 9.25 to 86.41 pg/mL, and interassay CVs were between 5.70 and 6.61% at concentrations of 7.3 to 118.51 pg/mL. The standard curve was highly reproducible (M slope = -0.70 and M-r = 0.99). Method accuracy, determined by spike recovery, and linearity, determined by serial dilution, were 99.20 and 92.80%, respectively. Values from matched serum and saliva samples showed strong linear relationships. The assay captured near 99.09% of the range of individual differences in boys' (N = 90) and girls' (N = 85), ages 8-12, AM and PM salivary testosterone levels. This assay can be easily applied to the investigation of testosterone-behavior relations in the context of studies on child health and development. It may help many child development researchers improve or expand their research activities. (C) 1999 Academic Press.