Peri-insular presence of collagenase during islet isolation procedures.
Peri-insular presence of collagenase during islet isolation procedures.
复制标题
胰岛分离过程中岛周存在胶原酶。
DOI：
10.1016/0022-4804(92)90097-j
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
R. van Schilfgaarde
中科院分区：
文献类型：
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作者：
P. T. van Suylichem;G. Wolters;R. van Schilfgaarde
据报道，通过导管胶原酶施用和随后的胰腺消化比通过切碎的组织胶原酶消化技术获得更高的胰岛产量。然而，胰岛分离的确切机制尚不清楚。本研究旨在了解导管内注射胶原酶产生良好效果的潜在机制。为此，我们研究了较高的产量是否可以通过与切碎的组织消化期间相比，导管应用后胰腺组织中胶原酶的不同分布来解释。印度墨水用于模拟和可视化几个物种的胰腺组织切片中胶原酶的分布。在导管应用后和切碎的组织胶原酶消化期间，在胰岛周围甚至胰岛内均观察到墨水颗粒。因此，胶原酶酶不限于外分泌组织区室与任何技术。鉴于这一观察结果，我们在大鼠配对实验中比较了两种技术在胰岛分离程序中的功效。这两种技术得到了类似的胰岛产量，据报道，获得与导管胶原酶的方法。然而，使用任一技术，胰岛产量仅为胰腺内分泌体积的约50%，表明已经发生了胰岛组织的大量损失。我们的结论是，无论胶原酶给药途径如何，在胰岛分离过程中，胶原酶都存在于岛周间隙中。鉴于两种方法在大鼠中具有相似的胰岛产量，并且胶原酶消化本身与胰岛组织的损失相关，这是相关的。
Reportedly, higher islet yields are obtained by ductal collagenase administration and subsequent digestion of the pancreas than by the chopped tissue collagenase digestion technique. However, the exact mechanism of islet isolation is not known. This study aims to understand the underlying mechanism of a favorable effect of ductal collagenase administration. To this end, we investigated if the higher yields can be explained by a different distribution of the collagenase enzymes in the pancreatic tissue after ductal application as compared to during chopped tissue digestion. India ink was used to mimic and visualize the distribution of collagenase in histological sections of pancreases of several species. Ink particles were seen around and even within the islets both after ductal application and during chopped tissue collagenase digestion. Thus, collagenase enzymes are not restricted to the exocrine tissue compartment with either technique. In view of this observation, we compared the efficacy of both techniques in islet isolation procedures in paired experiments in rats. Both techniques gave similar islet yields to those reportedly obtained with the ductal collagenase method. However, with either technique, the islet yield was only approximately 50% of the endocrine volume of the pancreas, indicating that a substantial loss of islet tissue had occurred. We conclude that, irrespective of the route of collagenase administration, collagenase enzymes are present in the peri-insular space during islet isolation procedures. This is pertinent in view of the finding that both methods have similar islet yields in rats and that collagenase digestion, as such, is associated with loss of islet tissue.