Sexual reproduction increases the possibility that Phytophthora capsici will develop resistance to dimethomorph in China
Sexual reproduction increases the possibility that Phytophthora capsici will develop resistance to dimethomorph in China
复制标题
有性繁殖增加了中国辣椒疫霉对烯酰吗啉产生抗性的可能性
DOI：
10.1111/ppa.12220
复制
发表时间：
2014-12-01
期刊：
影响因子：
2.7
通讯作者：
Liu, X.
中科院分区：
文献类型：
--
作者：
Bi, Y.;Hu, J.;Liu, X.
2006 - 2013年，从我国32个省、自治区、直辖市无羧酸酰胺类杀菌剂暴露史的辣椒疫病菌（Phytophthora capsici）中分离到1511株辣椒疫霉菌株。所有1511株测定交配型和403测定烯酰吗啉（DMM）和甲霜灵的敏感性。DMM EC 50值范围为0126 - 0339 gmL（-1）。A1和A2交配型均在4个省的同一个猪场检出，比例为1：1。大多数菌株对甲霜灵敏感，但少数菌株表现出中等抗性或抗性。通过对337个离体杂交或自交后代的DMM抗性和敏感性分离分析，表明辣椒疫霉对DMM的抗性受2对显性基因控制。来自杂交的18个后代在DMM敏感性和适应性上不同。一些后代与亲本分离物一样适合。考虑到交配型的分布以及有性繁殖的潜力、两个显性基因对抗性的控制以及杂交后代的适合度，中国辣椒疫霉群体发展DMM抗性的风险是巨大的。
A total of 1511 isolates of Phytophthora capsici were collected from farms with no history of exposure to the carboxylic acid amide (CAA) fungicides in 32 provinces in China during 2006 to 2013. All 1511 isolates were assayed for mating type and 403 were assayed for sensitivity to dimethomorph (DMM) and metalaxyl. The DMM EC50 values ranged from 0126 to 0339gmL(-1). Both A1 and A2 mating types were detected on the same farms in four provinces and with a 1:1 ratio. Most isolates were sensitive to metalaxyl but a few exhibited intermediate resistance or resistance to metalaxyl. The segregation of DMM resistance and sensitivity among 337 progeny obtained from hybridization or self-crossing in vitro indicated that the resistance of P.capsici to DMM is controlled by two dominant genes. Eighteen progeny that were derived from hybridization differed in DMM sensitivity and in fitness. Some progeny were as fit as parental isolates. Given the distribution of mating types and therefore the potential for sexual reproduction, the control of resistance by two dominant genes, and the fitness of hybrid progeny, the risk of P.capsici populations developing DMM resistance in China is substantial.