APPEARANCE OF WATER CHANNELS IN XENOPUS OOCYTES EXPRESSING RED-CELL CHIP28 PROTEIN
APPEARANCE OF WATER CHANNELS IN XENOPUS OOCYTES EXPRESSING RED-CELL CHIP28 PROTEIN
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DOI：
10.1126/science.256.5055.385
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发表时间：
1992-04-17
期刊：
影响因子：
56.9
通讯作者：
AGRE, P
中科院分区：
文献类型：
--
作者：
PRESTON, GM;CARROLL, TP;AGRE, P
水通过专门的通道快速穿过红细胞（RBC）和肾小管的质膜。虽然对水有选择性，但这些通道的分子结构是未知的。CHIP 28蛋白是哺乳动物红细胞和肾近端小管中丰富的整合膜蛋白，属于功能未知的膜蛋白家族。非洲爪蟾卵母细胞显微注射在体外转录的CHIP 28 RNA表现出增加渗透水的渗透性，这是可逆的抑制氯化汞，一种已知的水通道抑制剂。因此，CHIP 28可能是膜水通道的功能单元。
Water rapidly crosses the plasma membrane of red blood cells (RBCs) and renal tubules through specialized channels. Although selective for water, the molecular structure of these channels is unknown. The CHIP28 protein is an abundant integral membrane protein in mammalian RBCs and renal proximal tubules and belongs to a family of membrane proteins with unknown functions. Oocytes from Xenopus laevis microinjected with in vitro-transcribed CHIP28 RNA exhibited increased osmotic water permeability; this was reversibly inhibited by mercuric chloride, a known inhibitor of water channels. Therefore it is likely that CHIP28 is a functional unit of membrane water channels.