An inhibitor of apoptosis protein (EsIAP1) from Chinese mitten crab Eriocheir sinensis regulates apoptosis through inhibiting the activity of EsCaspase-3/7-1
An inhibitor of apoptosis protein (EsIAP1) from Chinese mitten crab Eriocheir sinensis regulates apoptosis through inhibiting the activity of EsCaspase-3/7-1
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DOI：
10.1038/s41598-019-56971-1
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发表时间：
2019-12-31
影响因子：
4.6
通讯作者：
Song, Linsheng
中科院分区：
文献类型：
--
作者：
Qu, Chen;Sun, Jiejie;Song, Linsheng
凋亡抑制蛋白（IAPs）通过抑制半胱天冬酶活性和介导免疫反应来维持细胞增殖和细胞死亡之间的平衡。在本研究中，从中华绒螯蟹（Eriocheir sinensis）中鉴定出一种IAP同源物（命名为EsIAP1）。EsIAP1由451个氨基酸组成，包含两个杆状病毒IAP重复（BIR）结构域，具有保守的Cx2Cx6Wx3Dx5Hx6C基序。EsIAP1 mRNA在各种组织中均有表达，其在血细胞中的表达水平在脂多糖刺激后12 - 48小时显著升高（p < 0.01）。在血细胞中，EsIAP1蛋白主要分布在细胞质中。重组EsCaspase - 3/7 - 1对底物Ac - DEVD - pNA的水解活性在与rEsIAP1孵育后降低。此外，rEsIAP1在体外可直接与rEsCaspase - 3/7 - 1结合。在EsIAP1被dsRNA干扰后，EsCaspase - 3/7 - 1的mRNA表达和水解活性显著增加，与dsGFP组相比分别增加了2.26倍（p < 0.05）和1.71倍（p < 0.05）。这些结果共同表明，EsIAP1可能通过调节中华绒螯蟹中EsCaspase - 3/7 - 1的活性在凋亡途径中发挥重要作用。
Inhibitor of apoptosis proteins (IAPs) maintain the balance between cell proliferation and cell death by inhibiting caspase activities and mediating immune responses. In the present study, a homolog of IAP (designated as EsIAP1) was identified from Chinese mitten crab Eriocheir sinensis. EsIAP1 consisted of 451 amino acids containing two baculoviral IAP repeat (BIR) domains with the conserved Cx2 Cx6 Wx3 Dx5 Hx6 C motifs. EsIAP1 mRNA was expressed in various tissues and its expression level in hemocytes increased significantly (p < 0.01) at 12-48 h after lipopolysaccharide stimulation. In the hemocytes, EsIAP1 protein was mainly distributed in the cytoplasm. The hydrolytic activity of recombinant EsCaspase-3/7-1 against the substrate Ac-DEVD-pNA decreased after incubation with rEsIAP1. Moreover, rEsIAP1 could directly combine with rEsCaspase-3/7-1 in vitro. After EsIAP1 was interfered by dsRNA, the mRNA expression and the hydrolytic activity of EsCaspase-3/7-1 increased significantly, which was 2.26-fold (p < 0.05) and 1.71-fold (p < 0.05) compared to that in the dsGFP group, respectively. These results collectively demonstrated that EsIAP1 might play an important role in apoptosis pathway by regulating the activity of EsCaspase-3/7-1 in E. sinensis.