Prenatal and early-life diesel exhaust exposure causes autism-like behavioral changes in mice.
Prenatal and early-life diesel exhaust exposure causes autism-like behavioral changes in mice.
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DOI：
10.1186/s12989-018-0254-4
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发表时间：
2018-04-20
影响因子：
10
通讯作者：
Costa LG
中科院分区：
文献类型：
--
作者：
Chang YC;Cole TB;Costa LG
近几十年来，自闭症谱系障碍（ASD）的患病率不断上升，引发了人们越来越多地努力了解环境风险因素所起的作用，以此来解决这一广泛的公共卫生问题。一些流行病学研究表明，发育暴露于交通相关的空气污染和ASD风险增加之间存在关联。在啮齿动物模型中，数量有限的研究表明，发育暴露于环境超细颗粒物或柴油机废气（DE）可导致与轻度ASD一致的行为表型。我们进行了一系列的实验，以确定是否发育DE暴露诱导小鼠ASD相关的行为。C57 Bl/6 J小鼠从胚胎第0天至出生后第21天暴露于250-300 μg/m3 DE或过滤空气（FA）作为对照。发育期暴露于DE的小鼠在ASD行为的所有三个标志性类别中表现出缺陷：在相互作用和社会偏好测试中减少社会互动，在T-迷宫和大理石埋藏测试中增加重复行为，以及通过测量隔离诱导的超声波发声和对社会气味的反应来评估减少或改变沟通。这些研究结果表明，暴露于与交通相关的空气污染，特别是与柴油燃料燃烧相关的空气污染，可导致小鼠ASD相关的行为变化，并引起对空气污染作为人类ASD发病因素的关注。本文的在线版本（10.1186/s12989-018-0254-4）包含补充材料，可供授权用户使用。
Escalating prevalence of autism spectrum disorders (ASD) in recent decades has triggered increasing efforts in understanding roles played by environmental risk factors as a way to address this widespread public health concern. Several epidemiological studies show associations between developmental exposure to traffic-related air pollution and increased ASD risk. In rodent models, a limited number of studies have shown that developmental exposure to ambient ultrafine particulates or diesel exhaust (DE) can result in behavioral phenotypes consistent with mild ASD. We performed a series of experiments to determine whether developmental DE exposure induces ASD-related behaviors in mice. C57Bl/6J mice were exposed from embryonic day 0 to postnatal day 21 to 250–300 μg/m3 DE or filtered air (FA) as control. Mice exposed developmentally to DE exhibited deficits in all three of the hallmark categories of ASD behavior: reduced social interaction in the reciprocal interaction and social preference tests, increased repetitive behavior in the T-maze and marble-burying test, and reduced or altered communication as assessed by measuring isolation-induced ultrasonic vocalizations and responses to social odors. These findings demonstrate that exposure to traffic-related air pollution, in particular that associated with diesel-fuel combustion, can cause ASD-related behavioral changes in mice, and raise concern about air pollution as a contributor to the onset of ASD in humans. The online version of this article (10.1186/s12989-018-0254-4) contains supplementary material, which is available to authorized users.