The effect of light conditions on interpreting oil composition engineering in Arabidopsis seeds
The effect of light conditions on interpreting oil composition engineering in Arabidopsis seeds
复制标题
DOI：
10.1002/pld3.67
复制
发表时间：
2018-06-01
期刊：
影响因子：
3
通讯作者：
Bates, Philip D.
中科院分区：
文献类型：
--
作者：
Karki, Nischal;Bates, Philip D.
拟南芥（Arabidopsis thaliana）是目前开发和利用最多的模式植物。特别是，它是概念验证种子油工程研究的优秀模型，因为它积累了约37%的种子油（按重量计），并且它与重要的豆科油料作物密切相关。拟南芥可以在包括连续光照在内的各种条件下生长;然而，光照量与总种子油积累密切相关。此外，许多试图在拟南芥中设计新的种子油脂肪酸组合物的尝试已经报道了油积累的显著减少;然而，与非转基因对照相比的相对减少在文献中变化很大。通过一系列试验，系统分析了光照条件（包括昼夜周期和连续光照，以及不同光照强度）对3个不同的产生新型羟基脂肪酸的转基因株系和它们的非转基因背景之间种子油相对积累的影响。测量每粒种子的含油量和含油量占种子重量的百分比。我们的研究结果表明，转基因株系和非转基因对照之间的种子油的相对量取决于光照条件和油含量测量的类型。此外，光照条件影响各种转基因品系之间新脂肪酸的相对积累。因此，在将工程策略转移到作物植物中之前，应根据选择的生长和测量条件来考虑新的脂肪酸概念验证工程策略在拟南芥种子中油积累和脂肪酸组成方面的成功。
Arabidopsis thaliana is the most developed and utilized model plant. In particular, it is an excellent model for proof-of-concept seed oil engineering studies because it accumulates approximately 37% seed oil by weight, and it is closely related to important Brassicaceae oilseed crops. Arabidopsis can be grown under a wide variety of conditions including continuous light; however, the amount of light is strongly correlated with total seed oil accumulation. In addition, many attempts to engineer novel seed oil fatty acid compositions in Arabidopsis have reported significant reductions in oil accumulation; however, the relative reduction from the nontransgenic controls varies greatly within the literature. A set of experiments were conducted to systematically analyze the effect of light conditions (including day/night cycle vs. continuous light, and different light intensities) on the relative accumulation of seed oil between three different transgenic lines producing novel hydroxy fatty acids and their nontransgenic background. Oil content was measured per seed and as a percentage of seed weight. Our results indicate the relative amount of seed oil between transgenic lines and nontransgenic controls is dependent on both the light conditions and the type of oil content measurement utilized. In addition, the light conditions effect the relative accumulation of the novel fatty acids between various transgenic lines. Therefore, the success of novel fatty acid proof-of-concept engineering strategies on both oil accumulation and fatty acid composition in Arabidopsis seeds should be considered in light of the select growth and measurement conditions prior to moving engineering strategies into crop plants.