Orientation of avidin molecules immobilized on COOH-modified SiO2/Si(100) surfaces
Orientation of avidin molecules immobilized on COOH-modified SiO2/Si(100) surfaces
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DOI：
10.1016/j.cplett.2005.11.060
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发表时间：
2006-02-15
影响因子：
2.8
通讯作者：
Urisu, T
中科院分区：
文献类型：
--
作者：
Misawa, N;Yamamura, S;Urisu, T
将抗生物素蛋白分子固定在COOH改性的SiO2/Si（100）表面上，其具有亚纳米级平坦度（均方根（rms）粗糙度;约0.1 nm），在基底表面上的COOH基团和抗生物素蛋白分子的NH 2基团之间形成共价键。亲和素固定化表面的结构进行了研究，原子力显微镜（AFM），椭圆偏振仪，红外（IR）反射吸收光谱，使用埋层金属基板（BML-IRRAS），和透射红外吸收光谱（TIRAS）。这些数据表明，亲和素分子是固定的2倍对称轴的四聚体几乎垂直于基板表面。(c)2005 Elsevier B. V.保留所有权利。
Avidin molecules were immobilized on a COOH-modified SiO2/Si(100) surface with subnano-level flatness (root-mean-square (rms) roughness;approximate to 0.1 nm) forming covalent bonds between the COOH groups on the substrate surface and the NH2 groups of avidin molecules. The structures of avidin-immobilized surfaces were investigated by atomic force microscopy (AFM), ellipsometry, and infrared (IR) reflection absorption spectroscopy using buried metal layer substrate (BML-IRRAS), and transmission IR absorption spectroscopy (TIRAS). These data have evidenced that the avidin molecules are immobilized with the 2-fold symmetry axis of the tetramer almost perpendicularly to the substrate surface. (c) 2005 Elsevier B.V. All rights reserved.