Feasibility of reusing the black liquor for enzymatic hydrolysis and ethanol fermentation
Feasibility of reusing the black liquor for enzymatic hydrolysis and ethanol fermentation
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黑液回用酶解乙醇发酵的可行性
DOI：
10.1016/j.biortech.2016.12.076
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发表时间：
2017
影响因子：
11.4
通讯作者：
Yuan Zhenhong
中科院分区：
文献类型：
--
作者：
Wang Wen;Chen Xiaoyan;Tan Xuesong;Wang Qiong;Liu Yunyun;He Minchao;Yu Qiang;Qi Wei;Luo Yu;Zhuang Xinshu;Yuan Zhenhong
碱法预处理过程中产生的黑液通常被认为是环境污染物。研究发现，纯碱木质素对纤维素酶解几乎没有抑制作用，因此研究了碱木质素作为缓冲液通过pH调节用于后续酶解和发酵的可行性。用乙酸将BL的pH值从13.23调节到4.80，碱木质素部分沉淀。它沉积在纤维素表面，通过阻断纤维素酶与纤维素的接触和吸附纤维素酶，对EHC产生负面影响。酸化后的发酵上清液对EHC几乎没有影响，但对乙醇发酵有抑制作用。稀释4倍的蔗渣碱处理上清液和最后一次的洗涤废水对EHC和乙醇的产生没有抑制作用。为碱法预处理工艺的节水提供了思路。
The black liquor (BL) generated in the alkaline pretreatment process is usually thought as the environmental pollutant. This study found that the pure alkaline lignin hardly inhibited the enzymatic hydrolysis of cellulose (EHC), which led to the investigation on the feasibility of reusing BL as the buffer via pH adjustment for the subsequent enzymatic hydrolysis and fermentation. The pH value of BL was adjusted from 13.23 to 4.80 with acetic acid, and the alkaline lignin was partially precipitated. It deposited on the surface of cellulose and negatively influenced the EHC via blocking the access of cellulase to cellulose and adsorbing cellulase. The supernatant separated from the acidified BL scarcely affected the EHC, but inhibited the ethanol fermentation. The 4-times diluted supernatant and the last-time waste wash water of the alkali-treated sugarcane bagasse didn’t inhibit the EHC and ethanol production. This work gives a clue of saving water for alkaline pretreatment.