Lysophosphatidic acids and their substrate lysophospholipids in cerebrospinal fluid as objective biomarkers for evaluating the severity of lumbar spinal stenosis
Lysophosphatidic acids and their substrate lysophospholipids in cerebrospinal fluid as objective biomarkers for evaluating the severity of lumbar spinal stenosis
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DOI：
10.1038/s41598-019-45742-7
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发表时间：
2019-06-24
影响因子：
4.6
通讯作者：
Chikuda, Hirotaka
中科院分区：
文献类型：
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作者：
Hayakawa, Kentaro;Kurano, Makoto;Chikuda, Hirotaka
溶血磷脂在动物模型神经病理性疼痛的启动和维持中具有潜在的重要作用。本研究利用人体标本研究了腰椎管狭窄症(LSS)的临床严重程度与其脑脊液(CSF)水平之间的关系。我们前瞻性地确定了28名LSS患者和15名对照，这些患者患有特发性脊柱侧弯或无神经症状的膀胱癌。我们用液相色谱-串联质谱法对脑脊液中的脂蛋白进行了定量。我们评估了LSS(神经病理性疼痛症状问卷(NPSI)和苏黎世言语障碍问卷(ZCQ))的临床结果，并根据患者的严重程度将患者分为两组。检测到5种溶血磷脂酸(LPA)、9种溶血磷脂酰胆碱(LPC)和1种溶血磷脂酰肌醇(LPI)。LSS患者各型LPL的脑脊液水平均显著高于对照组。重度NPSI组患者的LPL水平(3种LPA和9种LPC)明显高于轻度组。重度ZCQ组患者的LPL水平也明显升高(4种LPA和9种LPC)。本研究表明LPLs的脑脊液水平与LSS的临床严重程度呈正相关。LPL是评估LSS严重程度的潜在生物标志物。
Lysophospholipids (LPLs) are known to have potentially important roles in the initiation and maintenance of neuropathic pain in animal models. This study investigated the association between the clinical severity of lumbar spinal stenosis (LSS) and the cerebrospinal fluid (CSF) levels of LPLs, using human samples. We prospectively identified twenty-eight patients with LSS and fifteen controls with idiopathic scoliosis or bladder cancer without neurological symptoms. We quantified LPLs from CSF using liquid chromatography-tandem mass spectrometry. We assessed clinical outcome measures of LSS (Neuropathic Pain Symptom Inventory (NPSI) and Zurich Claudication Questionnaire (ZCQ)) and categorized patients into two groups according to their severity. Five species of lysophosphatidic acid (LPA), nine species of lysophosphatidylcholine (LPC), and one species of lysophosphatidylinositol (LPI) were detected. The CSF levels of all species of LPLs were significantly higher in LSS patients than controls. Patients in the severe NPSI group had significantly higher LPL levels (three species of LPA and nine species of LPC) than the mild group. Patients in the severe ZCQ group also had significantly higher LPL levels (four species of LPA and nine species of LPC). This investigation demonstrates a positive correlation between the CSF levels of LPLs and the clinical severity of LSS. LPLs are potential biomarkers for evaluating the severity of LSS.