Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains.
Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains.
复制标题
DOI：
10.1093/nar/8.23.5845
复制
发表时间：
1980-12
影响因子：
14.9
通讯作者：
M. Baez;R. Taussig;J. Zazra;J. F. Young;P. Palese;A. Reisfeld;A. Skalka
中科院分区：
文献类型：
--
作者：
M. Baez;R. Taussig;J. Zazra;J. F. Young;P. Palese;A. Reisfeld;A. Skalka
测定了人流感病毒A/PR/8/34的NS基因的核苷酸序列，发现其与另一种人流感病毒A/Udorn/72和禽分离株A/FPV/罗斯托克/34的NS基因具有相同的长度（890个核苷酸）。两种人流感病毒的NS基因序列的比较显示出8.9%的差异，而禽流感病毒分离株的NS基因与人流感病毒株A/PR/8/34的NS基因的差异仅为8%。这三个基因之间的广泛的序列相似性不支持禽流感病毒株和人流感病毒株的NS基因之间的种特异性同源组的概念。A/PR/8/34 NS基因的一级序列与流感病毒NS基因可能编码两个重叠多肽的发现一致。此外，在A/PR/8/34病毒NS基因的负链RNA中发现了一个可能编码167个氨基酸的多肽的开放阅读框架。
The nucleotide sequence of the NS gene of the human influenza virus A/PR/8/34 was determined and found to be the same length (890 nucleotides) as the NS gene of another human influenza virus A/Udorn/72 and of the avian isolate A/FPV/Rostock/34. Comparison of the sequences of the NS genes of the two human influenza viruses shows an 8.9% difference whereas the NS gene of the avian isolate differs by only 8% from that of the human strain A/PR/8/34. The extensive sequence similarity among these three genes does not support the notion of species specific homology groups among NS genes of avian and human influenza virus strains. The primary sequence of the A/PR/8/34 NS gene is consistent with the findings that the influenza virus NS gene may code for two overlapping polypeptides. In addition, an open reading frame potentially coding for a polypeptide 167 amino acids in length was found in the negative strand RNA of the A/PR/8/34 virus NS gene.