Formation of microbial products by activated sludge in the presence of a metabolic uncoupler o-chlorophenol in long-term operated sequencing batch reactors
Formation of microbial products by activated sludge in the presence of a metabolic uncoupler o-chlorophenol in long-term operated sequencing batch reactors
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在长期运行的序批式反应器中，在代谢解偶联剂邻氯苯酚存在下，活性污泥形成微生物产物
DOI：
10.1016/j.jhazmat.2019.121311
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发表时间：
2019
影响因子：
13.6
通讯作者：
Jia-Shun Cao
中科院分区：
文献类型：
--
作者：
Fang Fang;Su-Na Wang;Ke-Yan Li;Jin-Yun Dong;Run-Ze Xu;Lu-Lu Zhang;Wen-Ming Xie;Jia-Shun Cao
代谢解偶联剂在生物废水处理系统中被广泛用于减少剩余污泥。然而，在有代谢解偶联剂存在的情况下，活性污泥形成的微生物产物，如胞外聚合物、聚羟基脂肪酸酯和溶解性微生物产物，仍未被揭示。在本研究中，针对长期运行情况，评估了一种代谢解偶联剂邻氯苯酚（oCP）对实验室规模序批式反应器（SBRs）中活性污泥产率降低以及微生物产物形成的影响。结果表明，当投加5、10、15和20 mg/L的oCP时，四个反应器中污泥产率的平均降低率分别为17.40%、25.80%、33.02%和39.50%。oCP的添加略微降低了SBRs中的污染物去除效率，减少了溶解性微生物产物的形成，但刺激了活性污泥中胞外聚合物和聚羟基脂肪酸酯的产生。此外，添加oCP后电子传递系统活性显著降低。对活性污泥的微生物群落分析表明，投加oCP导致SBRs中污泥的丰富度和多样性下降。希望本研究能为生物废水处理系统中降低污泥产率以及在有解偶联剂存在时活性污泥的行为提供有用信息。
Metabolic uncouplers are widely used for reducing excess sludge in biological wastewater treatment systems. However, the formation of microbial products, such as extracellular polymeric substances, polyhydroxyalkanoate and soluble microbial products by activated sludge in the presence of metabolic uncouplers remains unrevealed. In this study, the impacts of a metabolic uncoupler o-chlorophenol (oCP) on the reduction of activated sludge yield and formation of microbial products in laboratory-scale sequencing batch reactors (SBRs) were evaluated for a long-term operation. The results show the average reduction of sludge yield in the four reactors was 17.40%, 25.80%, 33.02% and 39.50%, respectively, when dosing 5, 10, 15, and 20 mg/L oCP. The.oCP addition slightly reduced the pollutant removal efficiency and decreased the formation of soluble microbial products in the SBRs, but stimulated the productions of extracellular polymeric substances and polyhydroxyalkanoate in activated sludge. Furthermore, the significant reduction of electronic transport system activity occurred after the oCP addition. Microbial community analysis of the activated sludge indicates dosing oCP resulted in a decrease of sludge richness and diversity in the SBRs. Hopefully, this study would provide useful information for reducing sludge yield in biological wastewater treatment systems and behaviors of activated sludge in the presence of uncouplers.