Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats
Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats
复制标题
食用植物中黄嘌呤氧化酶抑制剂的筛选及高山姜提取物对高尿酸血症大鼠的降尿酸作用
DOI：
10.1016/j.jff.2018.09.024
复制
发表时间：
2018-11-01
影响因子：
5.6
通讯作者：
Zhao, Mouming
中科院分区：
文献类型：
--
作者：
Lin, Lianzhu;Liu, Xuemei;Zhao, Mouming
本研究旨在利用非靶向化学计量学方法从具有保健作用的食用植物中筛选具有黄嘌呤氧化酶（XOD）抑制作用和抗氧化活性的潜在抗高尿酸血症药物（层次聚类分析和主成分分析），评价其体内降尿酸作用和机制，超滤UPLC-MS/MS鉴定关键的XOD抑制剂。高良姜提取物（GE）具有高含量的总酚和黄酮，以及双重XOI和抗氧化活性，通过抑制XOD活性、减轻氧化应激、下调URAT 1和GLUT 9蛋白表达、降低尿素氮和肌酐水平，对高尿酸血症大鼠具有明显的降尿酸和肾脏保护作用。GE可能是一种具有XOI和促尿酸排泄双重作用的高尿酸血症防治药物。从GE中鉴定出4种黄酮类化合物和8种二芳基庚烷类化合物为潜在的XOD抑制剂。高良姜素、山奈素和3-甲基-高良姜素是XOD的主要抑制剂，它们对XOI的贡献率为70.1%。
This study was aimed at screening a potential anti-hyperuricemia agent with dual xanthine oxidase (XOD) inhibitory effect (XOI) and antioxidant activity from edible plants with health-promoting effects using nontargeted chemometric analysis (hierarchical cluster analysis and principal component analysis), evaluating its in vivo hypouricemic effect and mechanism, and identifying the key XOD inhibitor using ultrafiltration UPLC-MS/MS. Rhizoma Alpiniae Officinarum extract (GE), which has high contents of total phenolics and flavonoids, as well as dual XOI and antioxidant activity, showed significant hypouricemic and renal protective effects on hyperuricemic rats through inhibiting XOD activity, alleviating oxidative stress, down-regulating URAT1 and GLUT9 protein expression, and reducing urea nitrogen and creatinine levels. GE could be a dual-acting agent with XOI and uricosuric effect for preventing and treating hyperuricemia. Four flavonoids and eight diarylheptanoids were identified as potential XOD inhibitors from GE. Galangin, kaempferide and 3-methoxyl-galangin contributing to 70.1% of XOI were the key XOD inhibitors.