A UNIQUE MECHANISM REGULATING GENE-EXPRESSION - TRANSLATIONAL INHIBITION BY A COMPLEMENTARY RNA TRANSCRIPT (MICRNA)
A UNIQUE MECHANISM REGULATING GENE-EXPRESSION - TRANSLATIONAL INHIBITION BY A COMPLEMENTARY RNA TRANSCRIPT (MICRNA)
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DOI：
10.1073/pnas.81.7.1966
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
INOUYE, M
中科院分区：
文献类型：
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作者：
MIZUNO, T;CHOU, MY;INOUYE, M
主要外膜蛋白OmpF和OmpC基因的表达受渗透调节。ompC基因座在高渗透压条件下双向转录，在ompC上游编码的174碱基转录物抑制了OmpF的产生，并大幅减少了OmpF mRNA的数量。这种RNA [mRNA干扰互补RNA (micRNA)]具有与ompF mRNA的5'末端区域互补的长序列。micRNA通过与ompF mRNA杂交明显抑制其翻译。这种RNA相互作用可能导致ompF基因转录的过早终止或ompF mRNA的不稳定或两者兼而有之。
The expression of the [Escherichia coli] genes for the major outer membrane proteins OmpF and OmpC are osmoregulated. The ompC locus is transcribed bidirectionally under conditions of high osmolarity and a 174-base transcript encoded upstream of ompC inhibits the OmpF production and to substantially reduce the amount of the ompF mRNA. This RNA [mRNA-interfering complementary RNA (micRNA)] has a long sequence that is complementary to the 5'' end region of the ompF mRNA. The micRNA apparently inhibits the translation of the ompF mRNA by hybridizing with it. This RNA interaction may cause premature termination of the transcription of the ompF gene or destabilization of the ompF mRNA or both.