Dosage Effect of Zinc Glycine Chelate on Zinc Metabolism and Gene Expression of Zinc Transporter in Intestinal Segments on Rat
Dosage Effect of Zinc Glycine Chelate on Zinc Metabolism and Gene Expression of Zinc Transporter in Intestinal Segments on Rat
复制标题
甘氨酸锌螯合物剂量对大鼠肠段锌代谢及锌转运蛋白基因表达的影响
DOI：
10.1007/s12011-015-0535-9
复制
发表时间：
2016-06-01
影响因子：
3.9
通讯作者：
Feng, Jie
中科院分区：
文献类型：
--
作者：
Huang, Danping;Hu, Qiaoling;Feng, Jie
锌在各种基本生物过程中起着重要作用。本研究旨在探讨甘氨酸螯合锌（Zn-Gly）对大鼠肠道锌代谢及锌转运蛋白基因表达的剂量效应。将30只4周龄SD大鼠随机分为5个治疗组。各组基础日粮中分别添加0、30、60、90和180 mg/kg的甘氨酸锌。试验1周后，血清锌和肝脏锌浓度随补锌水平的增加而线性增加，补锌水平为0 ~ 180 mg/kg。血清Cu-Zn SOD活性呈显著（P< 0.01）的二次曲线变化，在60 mg/kg时达到峰值。Zn-Gly对肝脏Cu-Zn SOD和AKP活性的影响在0 ~ 180 mg/kg范围内呈线性关系。在十二指肠，MT 1 mRNA随Zn-Gly水平的升高而上调，在180 mg/kg时达到峰值（P< 0.05）。随着锌水平的升高，十二指肠和空肠中Zip 4 mRNA的表达量显著降低（P< 0.05）。在空肠中，60 mg/kg组Zip 5 mRNA表达量显著高于其他组（P< 0.05）。十二指肠ZnT 1 mRNA随锌含量的增加而增加，180 mg/kg组显著高于对照组（P< 0.05）。在十二指肠中，添加60或90 mg Zn/kg增加PepT 1表达，但在空肠中，60 mg Zn/kg与0添加Zn没有差异。总之，饲料Zn-Gly对大鼠血清和肝脏锌水平以及Cu-Zn SOD和AKP活性的影响呈剂量依赖性。日粮Zn-Gly对MT 1、Zip 4、Zip 5和ZnT 1的表达有一定的影响，不同Zn-Gly负荷水平的肠段MT 1、Zip 4、Zip 5和ZnT 1表达不同。此外，Zn-Gly还能调节肠段PepT 1的表达。
Zinc plays an essential role in various fundamental biological processes. The focus of this research was to investigate the dosage effect of zinc glycine chelate (Zn-Gly) on zinc metabolism and the gene expression of zinc transporters in intestinal segments. A total of 30 4-week-old SD rats were randomized into five treatment groups. The basal diets for each group were supplemented with gradient levels of Zn (0, 30, 60, 90, and 180 mg/kg) from Zn-Gly. After 1-week experiment, the results showed that serum and hepatic zinc concentration were elevated linearly with supplemental Zn levels from 0 to 180 mg Zn/kg. Serum Cu-Zn SOD activities resulted in a significant (P< 0.01) quadratic response and reached the peak when fed 60 mg Zn/kg. There were linear responses to the addition of Zn-Gly from 0 to 180 mg Zn/kg on Cu-Zn SOD and AKP activities in the liver. In the duodenum, MT1 mRNA was upregulated with the increasing dietary Zn-Gly levels and reached the peak of 180 mg Zn/kg (P< 0.05). Zip4 mRNA expression was downregulated with the increasing zinc levels (P< 0.05) in both duodenum and jejunum. In the jejunum, Zip5 mRNA expression in 60 mg Zn/kg was higher compared with other groups (P< 0.05). ZnT1 mRNA in duodenum was numerically increased with the rising levels of zinc content and was significantly higher (P< 0.05) with 180 mg Zn/kg. In the duodenum, adding 60 or 90 mg Zn/kg increased PepT1 expression, but in the jejunum, 60 mg Zn/kg did not differ from 0 added Zn. In summary, there is a dose-dependent effect of dietary Zn-Gly on serum and hepatic zinc levels and the activities of Cu-Zn SOD and AKP on rats. Dietary Zn-Gly has a certain effect on MT1, Zip4, Zip5, and ZnT1 expression, which expressed differently in intestinal segments with different levels of Zn-Gly load. Besides, Zn-Gly also could regulate PepT1 expression in intestinal segments.