[Cell kinetics of oral squamous epithelial carcinomas. Determination of the proliferation index and apoptosis rate with the TUNEL method].
[Cell kinetics of oral squamous epithelial carcinomas. Determination of the proliferation index and apoptosis rate with the TUNEL method].
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DOI：
10.1007/s100060050069
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发表时间：
1998-09-01
期刊：
影响因子：
--
通讯作者：
Hyckel, P
中科院分区：
文献类型：
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作者：
Junghanel, A;Berndt, A;Hyckel, P
肿瘤生长与肿瘤细胞数量的倍增直接相关，这首先可由增殖增加引起，其次可由肿瘤细胞寿命的延长引起。目的是量化与口腔鳞状上皮细胞癌细胞学分级相关的细胞数量的增加和减少（细胞凋亡）。在41例不同分级的口腔鳞状上皮癌中发现了TUNEL法（TdT介导的dUTP缺口末端标记）和单克隆抗体MIB 1增殖活性的DNA特异性断裂（双链断裂）的证据。随着细胞学分级的进展，发生增殖活性的增加和凋亡活性的降低。G1期癌细胞凋亡率为13.5%，G2期癌细胞凋亡率为8.5%，G3期癌细胞凋亡率为6.1%。结果证实，在口腔鳞状细胞癌进展到更高恶性细胞表型的过程中，增殖活性增加以及细胞凋亡率降低实质上有助于肿瘤细胞数量的倍增，并表明在进展过程中发生程序性细胞死亡（细胞凋亡）的失控。然而，不同级别的凋亡率的高标准差反驳了个体预后意义。
Tumour growth is directly related to the multiplication of tumour cell numbers which can be caused, firstly, by increased proliferation and, secondly, by prolongation of the life span of tumour cells. The aim was the quantification of the increase and decrease (apoptosis) in cell numbers in relation to the cytological grading of oral squamous epithelial cell carcinoma. Proof of apoptosis-specific fragmentation of DNA (double-strand breaks) by the TUNEL method (TdT-mediated dUTP nick end labelling) and of proliferative activity by the monoclonal antibody MIB 1 was found in 41 oral squamous epithelial carcinomas of different grading. With progression of the cytological grading, an increase of proliferative activity and a decrease of apoptotic activity occurs. The apoptosis rates were 13.5% for G1 carcinomas, 8.5% for G2 carcinomas and 6.1% for G3 carcinomas. The results confirm that during progression to higher malignant cellular phenotypes of oral squamous carcinoma, increased proliferative activity as well as reduced apoptosis rates contribute substantially to the multiplication of tumour cell numbers and show that during progression a loss of control of programmed cell death (apoptosis) occurs. However, the high standard deviations of apoptosis rates within the different grades disprove an individual prognostic significance.