Non-IgE-mediated hypersensitivity induced by multivitamins containing Tween-80.
Non-IgE-mediated hypersensitivity induced by multivitamins containing Tween-80.
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由含有 Tween-80 的多种维生素引起的非 IgE 介导的超敏反应。
DOI：
10.1111/1440-1681.13089
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发表时间：
2019
影响因子：
2.9
通讯作者：
Cao Lei
中科院分区：
文献类型：
--
作者：
Mi Yan-Ni;Yan Ping-Ping;Yu Rui-Hong;Xiao Xue;Wang Jin;Cao Lei
多种维生素已被广泛使用多年。对多种维生素的不良反应，特别是过敏反应越来越值得注意。然而，超敏反应的分类是混乱的，触发因素是未知的。多种维生素包括两个小瓶，标记为1号瓶含有Tween - 80和2号瓶。不含吐温- 80的复合维生素作为对照。采用观察、ELISA和血压计检测比格犬和豚鼠的行为学、组胺、IgE和血压，用分光光度法和流式细胞术检测RBL - 2H3细胞的脱粒和凋亡情况。结果表明，与对照组、复合维生素2和不含吐温- 80的复合维生素相比，首次服用复合维生素和含有吐温- 80的复合维生素1后，狗出现了多器官类过敏症状，血压显著降低，血浆组胺浓度升高。在过敏反应试验中，豚鼠未表现出任何过敏反应症状，除卵清蛋白外，复合维生素和复合维生素1瓶组、复合维生素2瓶和不含吐温- 80的复合维生素组血浆组胺和IgE浓度没有变化。与对照组相比，Tween - 80组、复合维生素组和复合维生素瓶1组非抗原致敏RBL - 2H3细胞的β -己糖氨酸酶和组胺释放量以及细胞凋亡显著增加，且呈浓度依赖性。然而，复合维生素2和不含吐温- 80的复合维生素组没有变化。结果提示，复合维生素引起的过敏可能是类过敏反应，而不是过敏反应。多种维生素诱导的炎症因子释放是由Tween - 80通过非IgE介导的途径触发的。
Multivitamins have been widely used for years. Adverse reactions, especially hypersensitivity, to multivitamins are becoming noteworthy. However, the classification of hypersensitivity is confusing, and the trigger is unknown. Multivitamins consist of two vials labelled vial 1 containing Tween‐80 and vial 2. Multivitamins without Tween‐80 were used as a contrast. Behaviouristics, histamine, IgE, and blood pressure of beagle dogs and guinea‐pigs were investigated by observation, ELISA and sphygmomanometer, and degranulation and apoptotic of RBL‐2H3 cells were assayed by spectrophotometry and flow cytometry. The results showed that dogs suffered from multiorgan anaphylactoid symptoms, and dramatically decreased blood pressure, and high plasma concentrations of histamine after the first administration of multivitamins and multivitamins vial 1, which contains Tween‐80, compared to the control, multivitamins vial 2 or multivitamins without Tween‐80. In anaphylaxis assay, guinea‐pigs did not display any anaphylaxis symptoms and there were no changes in plasma histamine and IgE concentrations in the multivitamins and multivitamins vial 1 groups or in the multivitamins vial 2 and multivitamins without Tween‐80 groups except ovalbumin. Compared to the control, the release of β‐hexosaminidase and histamine, and the apoptosis of non‐antigen‐sensitized RBL‐2H3 cells significantly increased in the Tween‐80 and multivitamins and multivitamins vial 1 groups in a concentration‐dependent manner. However, there was no alteration in multivitamins vial 2 and multivitamins without Tween‐80 groups. The results indicate that the hypersensitivity induced by multivitamins may be anaphylactoid reaction, but not anaphylaxis. Multivitamin‐induced release of inflammatory factors is triggered by Tween‐80 through a non‐IgE‐mediated pathway.