FISH HYPERSENSITIVITY .1. INVITRO AND ORAL CHALLENGE RESULTS IN FISH-ALLERGIC PATIENTS
FISH HYPERSENSITIVITY .1. INVITRO AND ORAL CHALLENGE RESULTS IN FISH-ALLERGIC PATIENTS
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DOI：
10.1016/0091-6749(92)90381-b
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发表时间：
1992-03-01
影响因子：
14.2
通讯作者：
SAMPSON, HA
中科院分区：
文献类型：
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作者：
BERNHISELBROADBENT, J;SCANLON, SM;SAMPSON, HA
这项研究的目的是确定对一种鱼类过敏的患者是否可以安全食用其他鱼类。对11例具有ige介导的鱼类过敏史的特应性、食物过敏儿童和年轻人进行了10种鱼类的皮肤点刺试验。11例患者中有8例皮肤点刺试验（SPTs）对所有10条鱼均呈阳性，其余3例患者至少有2条鱼SPTs阳性。7名患者中只有1条鱼口腔感染呈阳性，1名患者口腔感染2种鱼，2名患者口腔感染3种鱼。一名患者对测试的任何鱼都没有反应。对SPTs中使用的10种鱼类中的9种的生和熟蛋白质提取物进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹分析。生鱼提取物中的一些蛋白质条带随着烹调而变性，形成高分子量的聚集体。对记录在案的鱼过敏患者的血清进行免疫印迹分析，结果表明，通过口腔攻击确定，IgE与临床未过敏患者的鱼蛋白带结合。在elisa抑制试验中，达到50%抑制所需的鱼抗原浓度对于患者临床过敏的鱼与临床耐受的鱼是相似的。SPT和ige特异性交叉反应的体外证据并不一定与症状性鱼过敏相关。此外，这些对鱼类过敏的患者能够食用一种或多种其他鱼类而没有不良过敏反应。
The purpose of this study was to determine whether patients allergic to one fish species can safely eat other fish species. Eleven atopic, food-allergic children and young adults with histories consistent with IgE-mediated fish hypersensitivity were skin prick tested to 10 fish species. Skin prick tests (SPTs) were positive to all 10 fish in eight of the 11 patients, and the remaining three patients had at least two positive fish SPTs. Positive oral challenges occurred to only one fish in seven of the patients, to two fish species in one patient, and to three fish species in two patients. One patient did not react to any of the fish tested. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses were performed on raw and cooked protein extracts from nine of the 10 fish species used in SPTs. Several protein bands in the raw-fish extracts appeared to denature with cooking and form high molecular weight conglomerates. Immunoblot analyses with sera from documented fish-allergic patients demonstrated specific IgE binding to protein bands from fish to which patients were not clinically allergic, as determined by oral challenge. In ELISA-inhibition assays, the concentration of fish antigen required to achieve 50% inhibition was similar for fish to which the patients were clinically allergic as compared to fish to which they were clinically tolerant. SPT and in vitro evidence of IgE-specific cross-reactivity does not necessarily correlate with symptomatic fish allergy. In addition, these fish-hypersensitive patients were able to consume one or more other fish species without adverse allergic reactions.