Contribution of circulating hemocytes to the regeneration of heavy ion beams (12C5+) irradiated hematopoietic organs in the silkworm, Bombyx mori, through the way of phagocytosis of injured cells after invasion
Contribution of circulating hemocytes to the regeneration of heavy ion beams (12C5+) irradiated hematopoietic organs in the silkworm, Bombyx mori, through the way of phagocytosis of injured cells after invasion
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DOI：
10.1016/j.dci.2005.08.003
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Watanabe, H
中科院分区：
文献类型：
--
作者：
Ling, E;Shirai, K;Watanabe, H
重离子束照射在肿瘤治疗中具有广阔的应用前景。我们以前用家蚕进行的研究表明，这种辐射会严重损害幼虫的造血器官，但它们会在以后再生。在离体照射中，将造血器官和与表皮相连的翅盘从表皮向造血器官定向照射，然后进行培养以排除循环血细胞，大多数血细胞死亡。少数血细胞因体外造血极低而逃避照射。几乎没有血细胞可以纳入BrdU在60小时后照射，与活的和增殖的血细胞也标记。在缺乏循环血细胞的情况下，器官中的辐射逃逸血细胞不足以清除所有死亡细胞，因为许多小的死亡体在照射后仍留在原位。照射幼虫造血器官（体内照射）后，在相同恢复时间内，仅发现少数凋亡细胞，大部分血细胞保持正常形态。许多hernocytes纳入BrdU测试时，在同一时间在体外照射，但这个数字低于对照器官测量。经荧光微珠标记的血细胞在照射前通过吞噬作用侵入体内受照射的造血器官，帮助逃逸的血细胞清除再生过程中的死细胞。根据组织培养中产生的疝细胞数量，再生造血器官的造血没有完全恢复到对照器官的水平。部分释放的血细胞发生了明显的凋亡，表明碳离子束照射对血细胞内部存在着影响深远的旁观者效应。这些结果表明，侵入的循环血细胞与辐射逃逸的血细胞一起，在体内通过吞噬损伤血细胞的方式参与了重离子束照射后造血器官的再生。(c)2005-爱思唯尔有限公司保留所有权利。
Heavy ion beam irradiation has promising effects on tumor therapy. Our previous study using the domesticated silkworm, Bombyx mori, showed that this irradiation could seriously damage larval hematopoietic organs but they would regenerate later. In the in vitro irradiation, most hemocytes died when hematopoietic organs and wing discs connected with epidermis were directionally irradiated from epidermis to hematopoietic organ and then cultured so as to exclude circulating hemocytes. A few hemocytes had escaped irradiation according to extremely low hematopoiesis in vitro. Almost no hemocytes could incorporate BrdU at 60 h after irradiation, with which living and proliferating hemocytes are also labeled. In the absence of circulating hemocytes, the irradiation-escaped hemocytes in the organs were not enough for cleaning all dead cells because lots of small dead bodies remained in situ post-irradiation. After irradiating hematopoietic organs in larvae (in vivo irradiation), only a few apoptotic cells were found when given the same length of recovery time, and most hemocytes maintained normal morphology. Many hernocytes incorporated BrdU when tested at the same time as the in vitro irradiation but this number was lower than that measured for control organs. Circulating hemocytes, labeled by fluorescent microbeads through phagocytosis before irradiation, were found to have invaded the in vivo irradiated hematopoietic organs where they help the irradiation-escaped hemocytes to clear dead cells in the process of regeneration. Hematopoiesis of the regenerated hematopoietic organs did not fully recover to the level of the control organs according to the number of hernocytes produced in tissue culture. Some of the released hemocytes obviously underwent apoptosis, suggesting a far-reaching bystander effect of carbon ion beams irradiation on hemocytes inside. From these results, it is suggested that, together with irradiation-escaped hemocytes, the invaded circulating hemocytes took part in the regeneration of heavy ion beams irradiated hematopoietic organs through the way of phagocytosis of injured hemocytes in vivo. (c) 2005-Elsevier Ltd. All rights reserved.