Accumulation of tetrodotoxin and 4,9-anhydrotetrodotoxin in cultured juvenile kusafugu Fugu niphobles by dietary administration of natural toxic komonfugu Fugu poecilonotus liver
Accumulation of tetrodotoxin and 4,9-anhydrotetrodotoxin in cultured juvenile kusafugu Fugu niphobles by dietary administration of natural toxic komonfugu Fugu poecilonotus liver
复制标题
DOI：
10.1016/j.toxicon.2008.02.017
复制
发表时间：
2008-06-01
期刊：
影响因子：
2.8
通讯作者：
Yamamori, Kunio
中科院分区：
文献类型：
--
作者：
Kono, Michiko;Matsui, Takashi;Yamamori, Kunio
将无毒养殖的草食河豚幼鱼投喂含有剧毒天然小野河豚肝脏的饲料（TTX 8.0 μ g /鱼/天，4,9-无氢TTX 3.7 μ g /鱼/天）至第30天，再投喂无毒饲料至第240天。在30 ~ 240天内，定期取样5 ~ 6条鱼，共6次，测定各组织中TTX和4,9-无氢TTX的含量。在实验期间，所有组织中累积的总TTX和4,9-无氢TTX均保持在给药剂量的70%，无显著变化。然而，肝脏中TTX含量在第30天占120 μ g（占给药量的50%），随后逐渐下降至50 μ g，直到第240天，而4,9-无氢TTX含量在所有实验期间都保持在40 μ g左右（占给药量的40%）。与肝脏相反，皮肤中TTX和4,9-无水TTX在第30天分别为40和5 μ g，然后逐渐增加到80和24 μ g，直到第240天。在所有试验期间，肠道内TTX和4,9-无氢TTX的含量分别保持在25和12 μ g。根据这些结果，我们假设在肝脏中积累的一部分TTX慢慢转移到皮肤。(c) 2008 Elsevier Ltd.版权所有。
Non-toxic cultured juvenile kusafugu Fugu niphobles were fed with a diet containing highly toxic natural komonfugu Fugu poecilonotus liver until the 30th day (8.0 mu g of TTX and 3.7 mu g of 4,9-anhydroTTX/fish/day), and then fed with a non-toxic diet until the 240th day. During the 30-240th day, five or six fish were periodically sampled six times, and the contents of TTX and 4,9-anhydroTTX in each tissue were determined. The total TTX and 4,9-anhydroTTX accumulated in all tissues tested was not significantly changed during the experimental period, both being kept: at 70% of administrated doses. However, in the liver, the TTX content accounted to be 120 mu g (50% of administrated) on the 30th day, and then it gradually decreased to 50 mu g until the 240th day, while 4,9-anhydroTTX content was kept at approximately 40 mu g (40% of administrated) during all the experimental periods. In contrast to the liver, in the skin, TTX and 4,9-anhydroTTX were 40 and 5 mu g, respectively, on the 30th day, and then gradually increased to 80 and 24 mu g, respectively, until the 240th day. In the intestine, TTX, and 4,9-anhydroTTX contents were kept at 25 and 12 mu g, respectively, during all the experimental periods. According to these results, we assumed that a part of TTX accumulated in the liver was slowly transferred to the skin. (c) 2008 Elsevier Ltd. All rights reserved.