Assessment of feline ehrlichiosis in central Spain using serology and a polymerase chain reaction technique
Assessment of feline ehrlichiosis in central Spain using serology and a polymerase chain reaction technique
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DOI：
10.1196/annals.1307.013
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Sainz, A
中科院分区：
文献类型：
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作者：
Aguirre, E;Tesouro, MA;Sainz, A
埃里希体属抗体和与埃里希体属（Ehrlichia spp.）此前在西班牙也曾检测到猫血细胞中的病毒。本研究的目的是评估是否存在抗E. canis，犬新单胞菌N. ristiopathy和A.在来自马德里（西班牙中部）的122份猫血清样本中检测到嗜吞噬细胞菌。此外，对这些猫的血样进行埃立克体属特异性单管巢式聚合酶链反应（PCR）。10.6%的猫血清E.犬的阳性率为2.4%。restitivine和4.9%的A.嗜吞噬细胞菌两个N Risticii阳性猫和1只动物对A.嗜吞噬细胞的E.犬。尽管这些血清学阳性结果，所有的血液样本进行PCR分析是阴性的。我们的研究结果表明，对涉及在西班牙猫埃里希体病的代理商的反应。血清学和PCR在诊断猫埃立克体病中的意义有待进一步研究。
Antibodies to Ehrlichia spp. and inclusion bodies compatible with Ehrlichia spp. in feline blood cells have been previously detected in Spain. The aim of this study was to assess the presence of antibodies to E. canis, N. risticii, and A. phagocytophilum in 122 feline serum samples from Madrid (central Spain). In addition, Ehrlichia genus-specific, one-tube, nested polymerase chain reaction (PCR) was performed from blood samples from these cats. Of the cats, 10.6% were seropositive for E. canis, 2.4% were positive for N. risticii, and 4.9% were seropositive for A. phagocytophilum. Two N. risticii-positive cats and one animal seropositive to A. phagocytophilum were also seropositive for E. canis. Despite these seropositive results, all the blood samples analyzed by PCR were negative. Our results demonstrate reactivity against agents implicated in feline ehrlichiosis in Spain. Further studies should be performed in order to clarify the significance of serology and PCR in the diagnosis of feline ehrlichiosis.