tRNA/mRNA Mimicry by tmRNA and SmpB in Trans-Translation.
tRNA/mRNA Mimicry by tmRNA and SmpB in Trans-Translation.
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DOI：
10.4061/2011/130581
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发表时间：
2011-01-05
影响因子：
2.3
通讯作者：
Himeno H
中科院分区：
文献类型：
--
作者：
Kurita D;Muto A;Himeno H
由于从mRNA到蛋白质的准确翻译对生存至关重要，细胞已经开发出翻译质量控制系统。细菌核糖体在截断的mRNA上被一个涉及tmRNA和SmpB的系统拯救，称为反翻译。在此，我们回顾了目前对翻译机制的理解。基于定向羟基自由基探测的结果，我们提出了一种新的反翻译过程中的分子模拟。除了这些化学方法外，生化和低温电镜研究已经揭示了反翻译多个阶段的结构和功能方面。这些密集的工作为研究核糖体中tmRNA/SmpB的动态提供了基础。
Since accurate translation from mRNA to protein is critical to survival, cells have developed translational quality control systems. Bacterial ribosomes stalled on truncated mRNA are rescued by a system involving tmRNA and SmpB referred to as trans-translation. Here, we review current understanding of the mechanism of trans-translation. Based on results obtained by using directed hydroxyl radical probing, we propose a new type of molecular mimicry during trans-translation. Besides such chemical approaches, biochemical and cryo-EM studies have revealed the structural and functional aspects of multiple stages of trans-translation. These intensive works provide a basis for studying the dynamics of tmRNA/SmpB in the ribosome.