Tissue-specific expression of rat sulfotransferase messenger RNAs.
Tissue-specific expression of rat sulfotransferase messenger RNAs.
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发表时间：
1998-06
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通讯作者：
Robert T. Dunn;Curtis D. Klaassen
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作者：
Robert T. Dunn;Curtis D. Klaassen
本研究检测了大鼠硫酸转移酶(SULT)mRNAs的组织分布，以评估每个组织在硫酸盐化过程中的相对贡献。所检测的SULT亚型是雄性显性的SULT1A1、SULT1C1和SULT1E2，雌性显性的SULT20/21、SULT40/41和SULT60，以及最近克隆的非性别依赖的SULT(SULT1B1)。根据底物偏好，硫磺分为两类：苯酚硫磺(SULT1A1、SULT1B1、SULT1C1和SULT1E2)和羟基类固醇硫磺(SULT20/21、SULT40/41和SULT60)。用[α-32P]dATP标记的针对sultmRNAs的寡核苷酸探针进行Northern印迹分析：肝、脑、肺、心、肠、肾、肾上腺、前列腺、睾丸、卵巢、子宫和脾组织中sultm RNA的表达。每个结果的组织表达水平被量化，并通过磷放射自显影分析与肝脏表达进行比较。SULT在肝、脑、肺、心、肠、肾、肾上腺、睾丸和脾中表达，SULT1C1在肝、肾和脾中表达，SULT1E2仅在肝脏和心脏中表达。以雌性为主的海沟表现出更有限的组织分布。SULT20/21和SULT60仅在肝脏和肾上腺有表达，而SULT40/41只在肝脏有表达。SULT1B1在雄性和雌性大鼠的组织中表达程度相似，在肝脏、肠道和肾脏中检测到SULT1B1。除SULT1A1在肺中大量表达外，SULT1B1在肠道中的表达水平较高，其他组织中SULT1A1的表达水平明显高于其他组织。这些研究表明，肝脏是表达多种酶的最多样化的器官，因此是参与硫酸盐化的最重要的器官。与肝脏相反，肝外组织表达特定的sultmRNAs，这可能对每个组织的生理作用很重要。
The present study examined the tissue distribution of rat sulfotransferase (SULT) mRNAs to assess the relative contribution of each tissue to the process of sulfation. The SULT isoforms examined were male-dominant SULTs (SULT1A1, SULT1C1, and SULT1E2), female-dominant SULTs (SULT20/21, SULT40/41, and SULT60), and the recently cloned, non sex-dependent SULT (SULT1B1). SULTs fall into two distinct classes based on substrate preference: phenol SULTs (SULT1A1, SULT1B1, SULT1C1, and SULT1E2) and hydroxysteroid SULTs (SULT20/21, SULT40/41, and SULT60). The following tissues were analyzed for SULT mRNA expression: liver, brain, lung, heart, intestine, kidney, adrenal, prostate, testes, ovary, uterus, and spleen by Northern blot analysis with [alpha-32P]dATP-labeled oligonucleotide probes specific for individual SULT mRNAs. Tissue expression levels of each SULT were quantified and compared with liver expression by phosphor-autoradiographic analysis. Male-dominant SULT expression was observed in many organs, where SULT1A1 was expressed in liver, brain, lung, heart, intestine, kidney, adrenal, testes, and spleen; SULT1C1 expression was observed in liver, kidney, and spleen; and SULT1E2 expression was observed only in liver and heart. The female-dominant SULTs exhibited a more limited tissue distribution. Expression of SULT20/21 and SULT60 was observed only in liver and adrenal gland, whereas SULT40/41 expression was observed only in liver. SULT1B1 was expressed to a similar extent in tissues of male and female rats and was detected in liver, intestine, and kidney. Expression of SULT mRNAs in liver was much higher than in other tissues, except for SULT1A1, which exhibited substantial expression in lung, and SULT1B1, which was expressed at relatively high levels in intestine. These studies indicate that liver is the most diverse organ with respect to expression of multiple SULT enzymes and is therefore the most significant organ involved in sulfation. In contrast to liver, extrahepatic tissues express specific SULT mRNAs, and this may be important for the physiological role of each tissue.