CONTACT INHIBITION OF DIVISION - INVOLVEMENT OF ELECTRICAL TRANSMEMBRANE POTENTIAL
CONTACT INHIBITION OF DIVISION - INVOLVEMENT OF ELECTRICAL TRANSMEMBRANE POTENTIAL
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DOI：
10.1002/jcp.1040820307
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发表时间：
1973-01-01
影响因子：
5.6
通讯作者：
TONGIER, M
中科院分区：
文献类型：
--
作者：
CONE, CD;TONGIER, M
对 3T3 和中国仓鼠卵巢 (CHO) 细胞培养物中同时有丝分裂活性、跨膜电势 (Em) 和细胞密度水平的测量表明，Emlevel 增加 5 至 6 倍与饱和密度下有丝分裂停滞的发生有关。这种上升发生在汇合单层细胞和孤立集落的内部区域，并且与达到汇合的速率无关。 Emrise 伴随着细胞内 Na 的大幅减少。饱和 CHO 单层切片的电子显微镜显示 46% 至 63% 的细胞表面紧密并列（<300 Å 间距）。接触抑制培养物的这些结果支持有丝分裂活性可能与 Emlevel 和相关离子浓度水平功能性耦合的假设。有人认为，有丝分裂的接触抑制可能是由于接触引起的表面离子传输活性改变而产生的细胞内 Na 减少后，有丝分裂必需 RNA 的合成减少所致。
Measurements of simultaneous mitotic activity, electrical transmembrane potential (Em), and cell density levels in both 3T3 and Chinese hamster ovary (CHO) cell cultures reveal that a 5‐ to 6‐fold increase in the Emlevel is associated with development of mitotic arrest at saturation densities. This rise occurs both in confluent monolayers and in interior areas of isolated colonies, and is independent of the rate at which confluence is attained. The Emrise is accompanied by a substantial decrease in intracellular Na. Electron microscopy of saturated CHO monolayer sections shows from 46 to 63% of the cell surfaces to be in close apposition (<300 Å spacing). These results for contact inhibited cultures support the hypothesis that mitotic activity may be functionally coupled with the Emlevel and associated ionic concentration levels. It is suggested that contact inhibition of mitosis may result from a reduction in synthesis of mitogenically essential RNA following a decrease in intracellular Na produced by contact‐induced alteration of surface ion‐transport activity.