Integration and excision of polyoma virus genomes.
Integration and excision of polyoma virus genomes.
复制标题
多瘤病毒基因组的整合和切除。
DOI：
10.1101/sqb.1980.044.01.064
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Dailey,L
中科院分区：
文献类型：
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作者：
Basilico,C;Zouzias,D;Della-Valle,G;Gattoni,S;Colantuoni,V;Fenton,R;Dailey,L
结果所用多瘤病毒转化细胞系均为来源于F2408大鼠细胞的克隆性分离株(Freeman等。1973年；Prasad等人。1976)感染后，在软琼脂培养基中培养。PY54系是野生型多瘤病毒转化的大鼠54细胞系，TSA 13、TSA H5和TSA H6A系由TSA多瘤病毒突变体(Fry 1965)转化而成。已经描述了这些生产线的生产方式(Basilo等人)。1979年)。转化后的表型在所有细胞中的表达均不依赖于温度。在实验开始前不久，在液体培养基或琼脂中重新克隆了这些细胞系。
RESULTSThe polyoma-virus-transformed cell lines used were all clonal isolates derived from F2408 rat cells (Freeman et al. 1973; Prasad et al. 1976) after infection and plating in soft-agar medium. The Py54 line is a Rat-54 line transformed by wild-type polyoma virus, and the tsA 13, tsA H5, and tsA H6A lines were transformed by the tsA polyoma virus mutant (Fried 1965). The modalities of production of these lines have already been described (Basilico et al. 1979). The expression of the transformed phenotype in all these cells was not temperaturedependent. The cell lines were recloned in liquid medium or in agar shortly before the beginning of the experiments.