The use of Chinese hamster ovary (CHO) cells in the study of ion channels
The use of Chinese hamster ovary (CHO) cells in the study of ion channels
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DOI：
10.1016/j.vascn.2004.08.008
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发表时间：
2005-05-05
影响因子：
1.9
通讯作者：
Shapiro, Mark S.
中科院分区：
文献类型：
--
作者：
Gamper, Nikita;Stockand, James D.;Shapiro, Mark S.
上皮样中国仓鼠卵巢（CHO）细胞系由T. T. 1957年的帕克。从那时起，CHO细胞已成为工业和科学中广泛使用的哺乳动物表达系统。本文讨论了CHO细胞生理学的不同特点以及使用这些细胞进行离子通道研究的具体方面;讨论的特点是CHO细胞的培养和转染，从他们的电生理记录的细节和应用研究的离子通道生理学和药理学。本文讨论的哺乳动物离子通道在CHO细胞中的成功重建的例子包括毒蕈碱乙酰胆碱受体的KCNQ通道调节的重建和上皮钠通道（ENaC）的阿米洛利敏感性的研究。(C)2005年爱思唯尔公司All rights reserved.
The line of epithelial-like Chinese hamster ovary (CHO) cells was initiated by T.T. Puck in 1957. Since then, CHO cells have become a widely used mammalian expression system in industry and science. This paper discusses the different features of CHO cell physiology as well as the specific aspects of using these cells for ion channel studies; among the discussed features are the culturing and transfection of CHO cells, details of electrophysiological recordings from them and applications for the study of ion channel physiology and pharmacology. Examples of successful reconstitution of mammalian ion channels in CHO cells discussed in the paper include reconstitution of KCNQ channel regulation by muscarinic acetylcholine receptors and the study of the amiloride-sensitivity of epithelial sodium channels (ENaC). (C) 2005 Elsevier Inc. All rights reserved.