Effect of seasonal high temperature on the immune response in Apostichopus japonicus by transcriptome analysis
Effect of seasonal high temperature on the immune response in Apostichopus japonicus by transcriptome analysis
复制标题
转录组分析季节性高温对仿刺参免疫反应的影响
DOI：
10.1016/j.fsi.2019.07.012
复制
发表时间：
2019-09-01
影响因子：
4.7
通讯作者：
Xu, Dongxue
中科院分区：
文献类型：
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作者：
Li, Chao;Fang, Huahua;Xu, Dongxue
仿刺参（Apostichopus japonicas）是我国养殖业的一个重要品种。然而，海参养殖也面临挑战，其中之一就是夏季高温。在本研究中，我们探讨了转录组表达谱与季节（四月，六月和七月）的肌肉组织中。japonicas.采样时自然海岸的温度分别为13 ℃、21 ℃和25 ℃。与APR组相比，JUL组表达谱的变化较JUN组明显。共有46个差异表达基因（DEG）参与先天免疫和获得性免疫，其中包括27个上调和19个下调基因。它们进一步被分为10个亚类：热休克，凝血级联反应，抗原加工和呈递，炎症反应，转运蛋白活性，免疫球蛋白，凝集素C，细胞粘附，活性氧（ROS）清除，凋亡和自噬。该研究将为深入了解冬小麦对季节性高温生理反应的分子机制提供理论依据。japonicas.特别是，了解季节性温度对物种的免疫影响对于野生和水产养殖种群的最佳管理实践至关重要。
The sea cucumber Apostichopus japonicas is a flourishing aquaculture species in China. However, there are challenges for sea cucumber aquaculture, one of which is the high temperature in summer. In this study, we explored the transcriptome expression profiles with seasons (APR, JUN and JUL) in the muscle tissue of A. japonicas. The temperature of the natural coast was 13 degrees C, 21 degrees C and 25 degrees C respectively when sampling. Compared with APR group, changes of expression profiles were more significant in JUL group than that in JUN group. A total of 46 differential expressed genes (DEGs) involved in both innate and adaptive immunity were highlighted, including 27 up-regulated and 19 down-regulated genes. They were further grouped into 10 sub-classes: heat shock, coagulation cascades, antigen processing and presentation, inflammatory response, transporter activity, immunoglobulin, lectin C, cell adhesion, reactive oxygen species (ROS) scavenging, apoptosis and autophagy. The study will offer deep insights of the molecular mechanisms underlying the physiological responses to seasonal high temperature in A. japonicas. Particularly, knowledge about the immunological effects of seasonal temperature on the species is critical for the optimal management practices for both wild and aquaculture populations.