Developmental role and auxin responsiveness of class III homeodomain leucine zipper gene family members in rice
Developmental role and auxin responsiveness of class III homeodomain leucine zipper gene family members in rice
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DOI：
10.1104/pp.108.118679
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发表时间：
2008-08-01
期刊：
影响因子：
7.4
通讯作者：
Nagato, Yasuo
中科院分区：
文献类型：
--
作者：
Itoh, Jun-Ichi;Hibara, Ken-Ichiro;Nagato, Yasuo
III类同源结构域亮氨酸拉链（III类HD-Zip）基因家族的成员是植物发育关键方面的中心调节因子。为了更好地了解5个III类HD-Zip基因在水稻发育中的作用，我们研究了它们的表达模式、异位表达表型和生长素反应性。4个基因，OSHB 1至OSHB 4，在一个本地化的域的拍摄顶端分生组织（SAM），叶原基的近轴细胞，叶缘，和木质部组织的维管束中表达。相反，OSHB 5的表达仅在韧皮部组织中观察到。异位表达抗microRNA 166的OSHB 3基因的植物表现出严重的缺陷，包括叶缘、芽和辐射状叶的异位产生。用生长素处理幼苗迅速诱导了SAM整个区域的异位OSHB 3表达，但在其他组织中没有。此外，这种异位表达的OSHB 3与叶起始缺陷。我们的研究结果表明，水稻III类HD-Zip基因具有保守的功能，其同系物在拟南芥（拟南芥），但也获得了特定的发展作用，在草或单子叶植物。此外，一些III类HD-Zip基因可能以生长素依赖的方式调节SAM中的叶起始过程。
Members of the Class III homeodomain leucine zipper ( Class III HD-Zip) gene family are central regulators of crucial aspects of plant development. To better understand the roles of five Class III HD-Zip genes in rice ( Oryza sativa) development, we investigated their expression patterns, ectopic expression phenotypes, and auxin responsiveness. Four genes, OSHB1 to OSHB4, were expressed in a localized domain of the shoot apical meristem (SAM), the adaxial cells of leaf primordia, the leaf margins, and the xylem tissue of vascular bundles. In contrast, expression of OSHB5 was observed only in phloem tissue. Plants ectopically expressing microRNA166-resistant versions of the OSHB3 gene exhibited severe defects, including the ectopic production of leaf margins, shoots, and radialized leaves. The treatment of seedlings with auxin quickly induced ectopic OSHB3 expression in the entire region of the SAM, but not in other tissues. Furthermore, this ectopic expression of OSHB3 was correlated with leaf initiation defects. Our findings suggest that rice Class III HD-Zip genes have conserved functions with their homologs in Arabidopsis ( Arabidopsis thaliana), but have also acquired specific developmental roles in grasses or monocots. In addition, some Class III HD-Zip genes may regulate the leaf initiation process in the SAM in an auxin-dependent manner.