Studies on the effect of quartz, bentonite and coal dust mixtures on macrophages in vitro.
Studies on the effect of quartz, bentonite and coal dust mixtures on macrophages in vitro.
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石英、膨润土和煤粉混合物对体外巨噬细胞影响的研究。
DOI：
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发表时间：
1978
影响因子：
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通讯作者：
M. Timár
中科院分区：
文献类型：
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作者：
Z. Adamis;M. Timár
体外测定了石英、膨润土、煤尘及其人工混合物对大鼠腹腔巨噬细胞TTC还原和细胞内外乳酸脱氢酶活性的影响。石英对细胞膜的破坏作用导致细胞外乳酸脱氢酶显著释放。膨润土没有引起细胞外酶的释放，这使我们相信这种粉尘的生物效应表现为细胞内乳酸脱氢酶活性的下降。石英和膨润土对TTC还原的抑制作用相同。在石英(60%)-膨润土(40%)粉尘混合物中，膨润土在体外和体内均能抑制石英的特异性作用。我们在煤-石英-膨润土混合物的体外实验中得到了同样的结果。我们的实验表明，将人工粉尘混合物和空气粉尘样品的生物效应进行比较是合理的，并证明同时进行各种检查可以更详细地说明矿物粉尘可能的生物效应。
The effect of quartz, bentonite and coal dusts as well as the effect of the artificial mixture of these dusts on TTC reduction and extra-and intra-cellular lactate dehydrogenase activity in peritoneal rat macrophages was determined in vitro. The cell-membrane-damaging effect of quartz caused a significant extracellular release of lactate dehydrogenase. Bentonite caused no extracellular enzyme release, which leads us to believe that the biological effect of this dust is shown by decrease in intra-cellular lactate dehydrogenase activity. TTC reduction was inhibited equally by quartz and qentonite. In mixtures of quartz (60%)-bentonite (40%) dust the specific effect of quartz was inhibited by bentonite in vitro and also in vivo. We obtained the same results with coal-quartz-bentonite dust mixtures in vitro. Our experiments show that comparison of the biological effects of artificial dust mixtures and airborne dust samples is justified, and prove that performing various examinations simultaneously give fuller particulars on the probable biological effect of mineral dusts.