Rational design of thiolated polyenes as trifunctional Raman reporter molecules in surface‐enhanced Raman scattering nanotags for cytokine detection in a lateral flow assay
Rational design of thiolated polyenes as trifunctional Raman reporter molecules in surface‐enhanced Raman scattering nanotags for cytokine detection in a lateral flow assay
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DOI：
10.1002/jbio.201960126
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发表时间：
2020-01
影响因子：
2.8
通讯作者：
Thomas Keller;Svetlana Brem;Vi Tran;Oliver Sritharan;D. Schäfer;S. Schlücker
中科院分区：
文献类型：
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作者：
Thomas Keller;Svetlana Brem;Vi Tran;Oliver Sritharan;D. Schäfer;S. Schlücker
吸附在贵金属纳米颗粒上的拉曼报告分子的特征振动光谱指纹被用于通过相应的表面增强拉曼散射（Sers）纳米标签标记的抗体识别靶蛋白。在这里，我们提出了模块化的合成硫醇化多烯与2至5个C C双键通过逐步Wittig反应引入。它们的电子和振动性质的实验表征是由密度泛函理论计算补充。通过使用硫醇化多烯作为具有多个热点的Au/Au核心/卫星超颗粒中的拉曼报告分子来产生高度Sers活性纳米标签。通过拉曼显微镜在硝酸纤维素测试条上进行基于双相Sers的侧向流测定来检测细胞因子IL-1β和IFN-γ。硫醇化多烯适用于免疫Sers应用，如即时检测以及细胞和组织成像。
The characteristic vibrational spectroscopic fingerprint of Raman reporter molecules adsorbed on noble metal nanoparticles is employed for the identification of target proteins by the corresponding surface‐enhanced Raman scattering (SERS) nanotag‐labeled antibodies. Here, we present the modular synthesis of thiolated polyenes with two to five C═C double bonds introduced via stepwise Wittig reactions. The experimental characterization of their electronic and vibrational properties is complemented by density functional theory calculations. Highly SERS‐active nanotags are generated by using the thiolated polyenes as Raman reporter molecules in Au/Au core/satellite supraparticles with multiple hot spots. The cytokines IL‐1β and IFN‐γ are detected in a duplex SERS‐based lateral flow assay on a nitrocellulose test strip by Raman microscopy. The thiolated polyenes are suitable for use in immuno‐SERS applications such as point‐of‐care testing as well as cellular and tissue imaging.