Evidence for melatonin synthesis in mouse and human bone marrow cells
Evidence for melatonin synthesis in mouse and human bone marrow cells
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DOI：
10.1034/j.1600-079x.2000.280401.x
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发表时间：
2000-05-01
影响因子：
10.3
通讯作者：
Maestroni, GJM
中科院分区：
文献类型：
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作者：
Conti, A;Conconi, S;Maestroni, GJM
最近，研究表明，近交系小鼠的松果体和血清褪黑素具有明确的昼夜节律。此外，褪黑素还参与许多免疫调节功能。其中，造血受褪黑素通过褪黑素诱导的阿片作用于骨髓基质细胞上的kappa-阿片受体的影响。因此，本研究旨在研究骨髓中褪黑素的存在，骨髓中产生免疫能力细胞。具体来说，我们旨在回答以下问题：骨髓细胞是否参与褪黑激素的合成？在目前的研究中，我们证明(1)骨髓细胞含有高浓度的褪黑激素；(2)骨髓细胞具有n -乙酰转移酶活性，它们表达编码羟基- o -甲基转移酶的mRNA；(3)长时间培养的骨髓细胞表现出高水平的褪黑激素。本研究的结果表明，小鼠和人的骨髓和骨髓细胞能够重新合成褪黑激素，褪黑激素可能具有细胞内和/或旁分泌功能。
Recently, it was demonstrated that inbred strains of mice have a clearcut circadian rhythm of pineal and serum melatonin. Moreover, it is known that melatonin is involved in many immunoregulatory functions. Among them, hematopoiesis is influenced by the action of melatonin via melatonin-induced opioids on kappa-opioid receptors, which are present on stromal bone marrow cells. Therefore, the present study was carried out to investigate the presence of melatonin in the bone marrow in which immunocompetent cells are generated. Specifically, we aimed at answering the following question: are bone marrow cells involved in melatonin synthesis? In the present study, we demonstrate that (1) bone marrow cells contain high concentrations of melatonin; (2) bone marrow cells have a N-acetyltransferase activity and they express the mRNA encoding hydroxy-O-methyltransferase and (3) bone marrow cells cultured for a prolonged period exhibited high levels of melatonin. Results presented here suggest that mouse and human bone marrow and bone marrow cells are capable of de novo synthesis of melatonin, which may have intracellular and/or paracrine functions.