Biodegradation of beta-cyfluthrin by Pseudomonas stutzeri strain S1
Biodegradation of beta-cyfluthrin by Pseudomonas stutzeri strain S1
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DOI：
10.1007/s10532-005-0211-4
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发表时间：
2005-12-01
期刊：
影响因子：
3.6
通讯作者：
Gopal, M
中科院分区：
文献类型：
--
作者：
Saikia, N;Das, SK;Gopal, M
近年来，β-氟氯氰菊酯[α-氰基-4-氟-3-苯氧基苄基-3（2，2-二氯乙烯基）-2，2-二甲基环丙烷羧酸酯]农药已在农业上用于防治影响茄科作物的鳞翅目害虫。合成拟除虫菊酯如高效氟氯氰菊酯的广泛使用导致了广泛的环境污染。本研究的目的是从土壤中分离细菌，并确定其降解β-氟氯氰菊酯的能力，并使用光谱法鉴定培养液中的中间体。采用富集培养的方法，从土壤中分离到一株高效氟氯氰菊酯降解菌。分离株S1的16 S rDNA序列与斯氏假单胞菌的16 S rDNA序列具有100%的同源性。最后，在β-氟氯氰菊酯降解过程中形成的产物被鉴定为氰基-4-氟-3-苯氧基苄基-3（2，2-二氯乙烯基）-2，2-二甲基环丙烷羧酸酯（M. W. 341）; 4-氟-3-苯氧基-α-氰基苄醇（M. W. 243)和3-（2，2-二氯乙烯基）-2，2-二甲基环丙烷甲酸（M. W. 208）。
beta-Cyfluthrin [alpha-cyano-4-fluoro-3-phenoxybenzyl-3(2,2-dichlorovinyl) - 2,2- dimethylcyclopropane carboxylate] pesticide has been in agricultural use in the recent years for controlling Lepidopteran pests affecting solanaceous crops. The extensive use of synthetic pyrethroids like beta-cyfluthrin has resulted in wide spread environmental contamination. The purpose of this study was to isolate bacteria from soil and to determine their ability to degrade beta-cyfluthrin and identify the intermediates in culture broth using spectroscopy. An aerobic bacterium capable of degrading beta-cyfluthrin was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate ( strain S1) had 100% identity to the sequence from Pseudomonas stutzeri. Finally products formed during degradation of beta-cyfluthrin have been identified as a cyano-4-fluoro-3-phenoxybenzyl- 3( 2, 2- dichlorovinyl) - 2,2- dimethylcyclopropane carboxylate (M. W. 341); 4-fluoro-3-phenoxy-alpha-cyanobenzyl alcohol ( M. W. 243) and 3( 2, 2- dichlorovinyl) - 2,2- dimethyl cyclopropanecarboxylic acid ( M. W. 208).